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| Status |
Public on Feb 09, 2011 |
| Title |
Roles of STAT3 and STAT5 in regulation of gene expression under Th17 differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Interleukin 2 (IL-2), a cytokine linked to human autoimmune diseases, limits IL-17 production. We show that deletion of Stat3 in T cells abrogates IL-17 production and attenuates autoimmunity associated with IL-2 deficiency. While STAT3 induces IL-17 and RORγt and inhibits Foxp3, IL-2 inhibited IL-17 independently of Foxp3 and RORγt. We found that STAT3 and STAT5 bound to multiple common sites across the Il17 genetic locus. The induction of STAT5 binding by IL-2 was associated with a reduction in STAT3 binding at these sites and the inhibition of associated active epigenetic marks. Titrating the relative activation of STAT3 and STAT5 modulated TH17 cell specification. Thus, the balance rather than the absolute magnitude of these signals determines the propensity of cells to make a key inflammatory cytokine.
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| Overall design |
The roles of STAT3 and STAT5 in regulation of gene expression under Th17 differentiation was investigated. Affymetrix Mouse Genome 430 2.0 Arrays were used to evaluate global gene expression.
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| Contributor(s) |
Yang X, Ghoreschi K, Steward-Tharp SM, Rodriguez-Canales J, Zhu J, Grainger JR, Hirahara K, Sun H, Wei L, Vahedi G, Kanno Y, O’Shea JJ, Laurence A |
| Citation(s) |
21278738, 23555662 |
| |
| Submission date |
Jan 11, 2011 |
| Last update date |
Feb 12, 2020 |
| Contact name |
Golnaz Vahedi |
| Organization name |
National Institutes of Health
|
| Department |
NIAMS
|
| Lab |
Lymphocyte Cell Biology Section
|
| Street address |
9000 Rockville Pike Bldg 10 Rm 13C101A
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| GSM652873 |
STAT3-wild type T cells, cultured 3 days in Th17 condition (expression) |
| GSM652874 |
STAT3-deficient T cells, cultured 3 days in Th17 condition (expression) |
| GSM652875 |
STAT5-wild type T cells, cultured 3 days in Th17 condition (expression) |
| GSM652876 |
STAT5-deficient T cells, cultured 3 days in Th17 condition (expression) |
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| This SubSeries is part of SuperSeries: |
| GSE26553 |
Opposing regulation of the Il17 locus through direct, reciprocal actions of STAT3 and STAT5 |
|
| Relations |
| BioProject |
PRJNA142285 |
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