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Series GSE266119 Query DataSets for GSE266119
Status Public on Apr 29, 2024
Title Influence of DNA-methylation at multiple stages of limb chondrogenesis
Organism Gallus gallus
Experiment type Methylation profiling by high throughput sequencing
Summary Limb development is a well-established model for understanding cell fate decisions, and the formation of skeletal elements is coordinated through a sequence of events that control chondrogenesis spatiotemporally. It has been established that epigenetic control participates in cartilage maturation. Our research has shown for the first time that the inhibition of DNA methylation in interdigital tissue with 5-azacytidine results in the formation of an ectopic digit. This discovery suggested that DNA methylation dynamics could regulate the fate of cells between chondrogenesis and cell death during autopod development. We sequenced WGBS libraries from in-vivo interdigital tissue treated with 5-azacytidine or DMSO to identify genome-wide methylation changes during the early stages of 5-azacytidine-induced chondrogenesis.
 
Overall design Whole Genome Bisulfite Sequencing libraries (WGBS) from interdigital tissue implanted beads, soaked in 250µM 5-azacytidine or DMSO for 4 hours.
 
Contributor(s) Pérez-Maldonado MA, González-González XA, Chimal-Monroy J, Marín-Llera JC
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Submission date Apr 29, 2024
Last update date Apr 30, 2024
Contact name Mario Alberto Perez-Maldonado
E-mail(s) marioperez@uadec.edu.mx
Phone 528661372195
Organization name UNAM
Department Institute of Biomedical Research
Lab Chimal-Monroy Lab
Street address Circuito Mario de la Cueva s/n
City Mexico City
State/province Mexico City
ZIP/Postal code 04510
Country Mexico
 
Platforms (1)
GPL26853 Illumina NovaSeq 6000 (Gallus gallus)
Samples (4)
GSM8240949 interdigital tissue implanted beads, DMSO_rep1
GSM8240950 interdigital tissue implanted beads, 5-Azacitidine_rep1
GSM8240951 interdigital tissue implanted beads, DMSO_rep2
Relations
BioProject PRJNA1105916

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Supplementary file Size Download File type/resource
GSE266119_RAW.tar 264.5 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA

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