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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 14, 2011 |
| Title |
Expression changes following siRNA knockdown of ADRM1 in ovarian cell line OAW42 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Knock-down of ovarian cancer amplification target ADRM1 leads to down regulation of GIPC1 and up-regulation of RECK.
Among 20q13-amplified genes in ovarian cancer, ADRM1 overexpression was the most highly correlated with amplification, and was significantly upregulated with respect to stage, recurrence and metastasis. In addition, overexpression of ADRM1 correlated significantly with shorter time to recurrence and overall survival. Herein, array-CGH and microarray expression of ovarian cancer cell lines, provides evidence consistent with the primary tumor data that ADRM1 is a 20q13 amplification target. Knock-down of ADRM1 in amplified ovarian cell line OAW42 results in down-regulation of growth factor GIPC1 and up-regulation of tumor-suppressor RECK RNA and protein. In our dataset of 141 ovarian primary tumors, ADRM1 overexpression significantly correlates with GIPC1 overexpression. In addition, there is a significant anticorrelation between ADRM1 overexpression and RECK expression. Further research is necessary to determine whether targeting knock-down of ADRM1 in 20q13-amplified ovarian cancers results in growth inhibition and tumor suppression via downstream targets GIPC1 and RECK.
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| Overall design |
ADRM1 siRNA treated OAW42 compared directly to untreated or nonspecific RNA treated OAW42
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| Contributor(s) |
Fejzo MS, Ginther C, Dering J, Anderson L, Venkatesan N, Karlan B, Slamon D |
| Citation(s) |
21432940 |
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| Submission date |
Jan 14, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Charles L Ginther |
| E-mail(s) |
ginther@ucla.edu
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| Phone |
310-586-2667
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| Organization name |
UCLA
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| Department |
Hem-Onc
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| Lab |
Slamon
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| Street address |
2825 Santa Monica Blvd, #200
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| City |
Santa Monica |
| State/province |
CA |
| ZIP/Postal code |
90404 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (2) |
| GSM655580 |
OAW42 siRNA ADRM1(B) vs OAW42 no added RNA (A) |
| GSM655581 |
OAW42 siRNA ADRM1(B) vs OAW42 nonspecific RNA (C) |
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| Relations |
| BioProject |
PRJNA136455 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26634_RAW.tar |
28.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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