NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE268921 Query DataSets for GSE268921
Status Public on Sep 24, 2024
Title Single Nucleus DNA Sequencing of Flow Sorted Archived Frozen and Formalin Fixed Paraffin Embedded Solid Tumors
Organism Homo sapiens
Experiment type Genome variation profiling by array
Summary We applied DNA content based flow cytometry methods to interrogate the genomes of clinical samples from 8 patients with ovarian cancer. These included 6 high grade serous ovarian carcinomas, a low grade serous carcinoma, and an endometriod carcinoma. Archived samples obtained from surgical resections from a University of California San Francisco (UCSF) tissue bank. Notably for this study we distinguished and sorted diploid and aneuploid tumors. We then profiled the the whole genome copy number for each sample. These data were used to provide standards for single cell analyses of the same tissues including FFPE samples.
 
Overall design We applied DNA content based flow sorting to isolate the nuclei of clonal populations from ovarian carcinoma biopsies. We coupled this strategy with oligonucleotide array CGH (aCGH) thereby obtaining high definition genomic profiles of clonal populations from each tumor.
 
Contributor(s) Zhou Y, Chen X, Chapman JS, Barrett MT
Citation missing Has this study been published? Please login to update or notify GEO.
NIH grant(s)
Grant ID Grant title Affiliation Name
UH3 CA238926 HRD-IA signatures in pancreatic ductal adenocarcinoma Mayo Clinic Arizona Michael T Barrett
Submission date Jun 03, 2024
Last update date Sep 25, 2024
Contact name Michael Thomas Barrett
E-mail(s) barrett.michael@mayo.edu
Phone 480-301-6736
Organization name Mayo Clinic Arizona
Department Molecular Pharmacology and Experimental Therapeutics
Street address 13400 East Shea Boulevard
City Scottsdale
State/province AZ
ZIP/Postal code 85259
Country USA
 
Platforms (1)
GPL9777 Agilent-021850 SurePrint G3 Human CGH Microarray (Feature Number version)
Samples (8)
GSM8302703 High grade serous ovarian carcinoma FF1
GSM8302704 High grade serous ovarian carcinoma FF2
GSM8302705 Low grade serous ovarian carcinoma FF3
Relations
BioProject PRJNA1119467

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE268921_RAW.tar 343.7 Mb (http)(custom) TAR (of TXT)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap