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| Status |
Public on Jun 22, 2011 |
| Title |
Human lupus netting neutrophils induce endothelial damage, infiltrate tissues and expose immunostimulatory molecules |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Our group has proposed that low-density granulocytes (LDGs) play an important role in lupus pathogenesis, as they can damage endothelial cells and synthesize increased levels of proinflammatory cytokines and type I interferons. LDGs have a heightened capacity to synthesize neutrophil extracellular traps (NETs). NETs from LDGs display increased levels of bactericidal and immunostimulatory proteins, such as the cathelicidin LL37 and externalize double-stranded DNA (dsDNA). Lupus netting LDGs have increased capacity to kill endothelial cells and expose IL-17. Through NETosis, lupus neutrophils stimulate plasmacytoid DCs to synthesize IFN-α. Our results further expand the potential pathogenic role of aberrant lupus neutrophils through a NET-mediated effect.
We used microarrays to analyze the gene expression of neutrophils in healthy and lupus patients, and of low-density granulocytes in lupus patients.
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| Overall design |
Human neutrophils and LDGs were isolated from PBMCs. RNA from healthy neutrophils, lupus neutrophils and lupus LDGs was extracted and processed for hybridization on Affymetrix microarrays.
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| Contributor(s) |
Villanueva E, Yalavarthi S, Berthier CC, Hodgin JB, Khandpur R, Lin AM, Rubin CJ, Zhao W, Olsen SH, Klinker M, Shealy D, Denny MF, Plumas J, Chaperot L, Kretzler M, Bruce AT, Kaplan M |
| Citation(s) |
21613614 |
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| Submission date |
Jan 31, 2011 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
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| Street address |
1150 W. Medical Center Dr, MSRB2
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL11670 |
Affymetrix Human Genome U133 Plus 2.0 Array [Hs133P_Hs_ENTREZG.cdf] |
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| Samples (29)
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| GSM664088 |
Healthy control neutrophil, 004 |
| GSM664089 |
Healthy control neutrophil, 005 |
| GSM664090 |
Healthy control neutrophil, 18 |
| GSM664091 |
Healthy control neutrophil, 29 |
| GSM664092 |
Healthy control neutrophil, 31 |
| GSM664093 |
Healthy control neutrophil, 32 |
| GSM664094 |
Lupus neutrophil, 007 |
| GSM664095 |
Lupus neutrophil, 009 |
| GSM664096 |
Lupus neutrophil, 011 |
| GSM664097 |
Lupus neutrophil, 013 |
| GSM664098 |
Lupus neutrophil, 015 |
| GSM664099 |
Lupus neutrophil, 2 |
| GSM664100 |
Lupus neutrophil, 9 |
| GSM664101 |
Lupus neutrophil, 11 |
| GSM664102 |
Lupus neutrophil, 21 |
| GSM664103 |
Lupus neutrophil, 23 |
| GSM664104 |
Lupus LDG, 006r |
| GSM664105 |
Lupus LDG, 008r |
| GSM664106 |
Lupus LDG, 010 |
| GSM664107 |
Lupus LDG, 012 |
| GSM664108 |
Lupus LDG, 014 |
| GSM664109 |
Lupus LDG, 1 |
| GSM664110 |
Lupus LDG, 7 |
| GSM664111 |
Lupus LDG, 8 |
| GSM664112 |
Lupus LDG, 10 |
| GSM664113 |
Lupus LDG, 12 |
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| Relations |
| BioProject |
PRJNA135917 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26975_RAW.tar |
137.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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