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Sample GSM664098 Query DataSets for GSM664098
Status Public on Jun 22, 2011
Title Lupus neutrophil, 015
Sample type RNA
 
Source name Neutrophil from lupus patient
Organism Homo sapiens
Characteristics tissue: peripheral blood
cell type: neutrophils
disease state: lupus
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Tri-pure (Roche), and further purified and concentrated using an Rneasy kit (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
 
Hybridization protocol Standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual).
Scan protocol GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
Description LupusNeutro015
Gene expression data from lupus neutrophils.
Data processing The data were analyzed using the GenePattern pipeline: the RMA method and Human Entrez Gene custom CDF annotation version 10 were used for the normalization, and Combat for batch correction.
 
Submission date Jan 31, 2011
Last update date Sep 01, 2016
Contact name Celine C Berthier
Organization name University of Michigan
Street address 1150 W. Medical Center Dr, MSRB2
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platform ID GPL11670
Series (1)
GSE26975 Human lupus netting neutrophils induce endothelial damage, infiltrate tissues and expose immunostimulatory molecules
Relations
Reanalyzed by GSE86362

Data table header descriptions
ID_REF
VALUE Log base 2 normalized expression value

Data table
ID_REF VALUE
1 3.690204351
2 3.407455212
9 6.847898764
12 2.777205605
13 3.016823666
14 5.021880168
15 3.079177867
16 5.143094341
18 8.38376305
19 10.47391103
20 5.758165308
21 5.209552696
22 6.651314784
23 6.650063415
24 3.590651113
25 5.300857598
26 4.719591163
27 4.551519178
28 5.066699558
29 6.869965703

Total number of rows: 17589

Table truncated, full table size 304 Kbytes.




Supplementary file Size Download File type/resource
GSM664098.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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