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| Status |
Public on Jun 27, 2024 |
| Title |
Controlling pericellular oxygen tension in cell culture reveals distinct breast cancer responses to low oxygen tensions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, the relationship between pericellular and gas phase oxygen concentration in cell culture was examined. To this end, we examined the influence of cellular oxygen consumption on primary human hepatocyte culture in physioxic (6% O2) and normoxic (18.6% O2) conditions. To understand how pericellular oxygen tension influenced hepatocyte biology in in vitro cell culture, we performed RNA-seq (this dataset).
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| Overall design |
RNA-seq was performed on primary human hepatocytes from the vial (uncultured), and hepatocytes cultured for 36h in normoxia (normoxia) or physioxia (physioxia). More details on methodology can be found in the source publication (https://doi.org/10.1002/advs.202402557)
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| Web link |
https://onlinelibrary.wiley.com/doi/full/10.1002/advs.202402557
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| Contributor(s) |
Rogers ZJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 27, 2024 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Zachary J Rogers |
| Organization name |
Northeastern University
|
| Department |
Chemical Engineering
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| Street address |
360 Huntington Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1129053 |
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