 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 15, 2024 |
| Title |
The single-cell assessment of transcriptiomic profile and chromain accessibility for the brain myeloid cells in rhesus macaques with SIV-induced encephalitis |
| Organism |
Macaca mulatta |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
Human immunodeficiency virus (HIV) is widely recognized for its striking impact on the immune system, and it also significantly impacts the central nervous system (CNS). The primary immune constituents in the brain, microglia and macrophages, are the target for HIV in people and the simian immunodeficiency virus (SIV) in nonhuman primates. This infection can lead to neurological dysfunction, known as HIV-associated neurocognitive disorder (HAND) and pathological entities such as HIV-induced encephalitis (HIVE). Given the gaps in our knowledge on how these cells respond in vivo to CNS infection, we performed single-cell multiomic sequencing, including gene expression and ATAC-seq on myeloid cells from the brains of two rhesus macaques with SIV-induced encephalitis (SIVE) as well as two uninfected controls. We used both RNA expression and chromatin accessibility to identify cell clusters. We found that the myeloid cell populations were significantly changed by SIVE, which differed greatly from those found in the uninfected brains. We categorized myeloid cells in the SIVE brains into two primary phenotypes: microglia-like and CAM-like, which were different from each other regarding trancriptomic profile and chromatin accessibility. We found that these microglia-like cells express high levels of chemoattractants for capable of recruiting highly the activated CAM-like cells to the site of infection/inflammation. Additionally, we observed a dramatic shift of upstream gene regulators and their targets in brain myeloid cells during SIVE. The transcription factors (TFs) highly enriched in SIVE-specific cell clusters were related to IRF/STAT and NF-κB mediated interferon and other proinflammatory cytokines production. Interestingly, some of these TF binding sites were also enriched in the open chromatin identified in the SIV DNA provirus in infected myeloid cells, indicating the immune activation in the host cells might also promote HIV/SIV gene expression and regulation . In summary, this study further uncover the trancriptome, gene regulaory events and potenital roles of differenet brain myeloid phenotypes in SIVE.
|
| |
|
| Overall design |
The 104T and 106T were uninfected control samples; the other two (21T and 34T) were in the SIV encephalitis (SIVE) group. One of the SIVE animals (21T) in the SIVE group was infected with the SIV for 92 days and another animal (34T) was infected for 49 days before necropsy. The 34T was also injected with methamphetamine for 60 days before SIV infection in a dose of 2.5mg/kg.
|
| |
|
| Contributor(s) |
Fox H, Morsey B, Lamberty B, Emanuel K, Niu M, Xu X |
| Citation(s) |
39372920 |
| |
| Submission date |
Jul 19, 2024 |
| Last update date |
Oct 16, 2024 |
| Contact name |
Xiaoke Xu |
| E-mail(s) |
x.xu@unmc.edu
|
| Organization name |
University of Nebraska Medical Center
|
| Street address |
42nd and Emile
|
| City |
Omaha |
| State/province |
Nebraska |
| ZIP/Postal code |
68198-7400 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL27943 |
Illumina NovaSeq 6000 (Macaca mulatta) |
|
| Samples (8)
|
| GSM8408910 |
Brain, SIVE, rep2, ATAC |
| GSM8408911 |
Brain, Uninfected, rep1, GEX |
| GSM8408912 |
Brain, Uninfected, rep2, GEX |
| GSM8408913 |
Brain, SIVE, rep1, GEX |
| GSM8408914 |
Brain, SIVE, rep2, GEX |
|
| Relations |
| BioProject |
PRJNA1137937 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE272669_RAW.tar |
9.6 Gb |
(http)(custom) |
TAR (of H5, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...