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| Status |
Public on Aug 01, 2011 |
| Title |
The effect of HP1-gamma deficiency on primordial germ cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We compared the gene expression profiles of the HP1 gamma -/- vs wild-type PGCs to examine the molecular mechanisms of HP1g in PGC development. Although HP1g is suggested to be involved in PGC proliferation, our microarray data indicated that HP1g does not directly control PGC cell-cycle progression by regulating cell cycle-related genes. However, many genes acting on differentiated tissues were up-regulated in the HP1g-/- PGCs.
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| Overall design |
The mVenus-positive cells from the E11.5 genital ridges were isolated by flow cytometry (JSAN, Bay Bioscience). The total RNA was then prepared from the sorted primordial germ cells (about 500-3,500 cells per embryo) of wild-type or HP1γ-/- embryos with an RNeasy micro kit (Qiagen). The RNA samples were pooled from 5 or 6 embryos of each genotype. Two independent pooled RNA samples of each genotypes were used to verify the reproducibility of the microarray analyses.
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| Contributor(s) |
Abe K, Naruse C, Kato T, Nishiuchi T, Saitou M, Asano M |
| Citation(s) |
21778144 |
| |
| Submission date |
Feb 14, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
Masahide Asano |
| E-mail(s) |
asano@kiea.m.kanazawa-u.ac.jp
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| Phone |
+81 76-265-2463
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| Fax |
+81 76-234-4240
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| Organization name |
Kanazawa University
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| Department |
Advanced Science Research Center,
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| Lab |
Division of Transgenic Animal Science
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| Street address |
13-1
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| City |
Kanazawa |
| State/province |
Ishikawa |
| ZIP/Postal code |
920-8640 |
| Country |
Japan |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA137345 |
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