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Series GSE27294 Query DataSets for GSE27294
Status Public on Aug 01, 2011
Title The effect of HP1-gamma deficiency on primordial germ cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We compared the gene expression profiles of the HP1 gamma -/- vs wild-type PGCs to examine the molecular mechanisms of HP1g in PGC development. Although HP1g is suggested to be involved in PGC proliferation, our microarray data indicated that HP1g does not directly control PGC cell-cycle progression by regulating cell cycle-related genes. However, many genes acting on differentiated tissues were up-regulated in the HP1g-/- PGCs.
Overall design The mVenus-positive cells from the E11.5 genital ridges were isolated by flow cytometry (JSAN, Bay Bioscience). The total RNA was then prepared from the sorted primordial germ cells (about 500-3,500 cells per embryo) of wild-type or HP1γ-/- embryos with an RNeasy micro kit (Qiagen). The RNA samples were pooled from 5 or 6 embryos of each genotype. Two independent pooled RNA samples of each genotypes were used to verify the reproducibility of the microarray analyses.
Contributor(s) Abe K, Naruse C, Kato T, Nishiuchi T, Saitou M, Asano M
Citation(s) 21778144
Submission date Feb 14, 2011
Last update date May 10, 2018
Contact name Masahide Asano
Phone +81 76-265-2463 
Fax +81 76-234-4240
Organization name Kanazawa University
Department Advanced Science Research Center,
Lab Division of Transgenic Animal Science
Street address 13-1
City Kanazawa
State/province Ishikawa
ZIP/Postal code 920-8640
Country Japan
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (4)
GSM674983 wild-type_E11.5_PGCs_rep1
GSM674984 wild-type_E11.5_PGCs_rep2
GSM674985 HP1 gamma -/-_E11.5_PGCs_rep1
BioProject PRJNA137345

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27294_RAW.tar 15.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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