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Series GSE27632 Query DataSets for GSE27632
Status Public on Mar 03, 2011
Title Copy number variation: the balance between gene dosage and expression in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Genome variation profiling by array
Summary Copy number variants (CNVs) reshape gene structure, modulate gene expression, and contribute to significant phenotypic variation. Previous studies have revealed CNV patterns in natural populations of Drosophila melanogaster and suggested that selection and mutational bias shape genomic patterns of CNV. While previous CNV studies focused on heterogeneous strains, here we established a number of second-chromosome substitution lines to uncover CNV characteristics when homozygous. The percentage of genes harboring CNVs is higher than found in previous studies. More CNVs are detected in homozygous than heterozygous substitution strains, suggesting the comparative genomic hybridization arrays underestimate CNV owing to heterozygous masking. We incorporated previous gene expression data collected from some of the same substitution lines to investigate relationships between CNV gene dosage and expression. Most genes present in CNVs show no evidence of increased or diminished transcription, and the fraction of such dosage-insensitive CNVs is greater in heterozygotes. More than 70% of the dosage-sensitive CNVs are recessive with undetectable effects on transcription in heterozygotes. A deficiency of singletons in recessive dosage-sensitive CNVs supports the hypothesis that most CNVs are subject to negative selection. On the other hand, relaxed purifying selection might account for the higher number of protein-protein interactions in dosage insensitive CNVs than in dosage-sensitive CNVs. Dosage-sensitive CNVs that are up-regulated and down-regulated coincide with copy number increases and decreases. Our results help clarify the relation between CNV dosage and gene expression in the D. melanogaster genome.
Overall design To determine copy number variation, the genomic DNA from five homozygous and two heterozygous second chromosome substitution lines were extracted and compared to another second chromosome substitution line. Gene expression levels can be referred to at Series GSE12191 (Lemos et al. (2008) PMID:18791071).
Contributor(s) Zhou J, Lemos B, Dopman EB, Hartl DL
Citation(s) 21979154
Submission date Mar 02, 2011
Last update date Aug 14, 2018
Contact name Jun Zhou
Phone 617-496-5540
Fax 617-496-5854
Organization name Harvard University
Department Organismic and Evolutionary Biology
Street address 16 Divinity Ave. Rm2112
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL6056 HartlLab Drosophilamelanogaster PCRproducts 22k
Samples (36)
GSM684998 A226
GSM684999 A227
GSM685000 A228
Affiliated with GSE12191
BioProject PRJNA138531

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Supplementary file Size Download File type/resource
GSE27632_RAW.tar 76.7 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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