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| Status |
Public on Sep 25, 2024 |
| Title |
Identification of essential transporter genes in the colorectal carcinoma cell model HCT116 by focused CRISPR/Cas9 KO screens. |
| Organisms |
Homo sapiens; blank sample |
| Experiment type |
Other
|
| Summary |
Transporters such as solute carriers (SLCs) are required to transport substrates such as nutrients or metals across cellular membranes. We here aimed to indentify which transporters are required for the proliferation of HCT116 WT and KO cells (SLC16A1, SLC16A3, SLC16A13, SLC25A28, SLC25A37 and SLC6A8 KOs).
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| Overall design |
Pooled CRISPR/Cas9 KO screen in standard media with proliferation readout.
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| Contributor(s) |
Wolf G, Onstein S, Uijttewaal E, Superti-Furga G |
| Citation missing |
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| |
| Submission date |
Sep 20, 2024 |
| Last update date |
Sep 25, 2024 |
| Contact name |
Giulio Superti-Furga |
| Organization name |
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
|
| Street address |
Lazarettgasse 14
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (2) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
| GPL27787 |
Illumina HiSeq 4000 (blank sample) |
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| Samples (53)
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| Relations |
| BioProject |
PRJNA1163356 |
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