|
| Status |
Public on Jun 14, 2005 |
| Title |
cMyb and vMyb in MCF7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in human MCF7 cells using recombinant adenovirus vectors. A hybrid construct, 3Mutc, which is a variant of c-Myb harboring three v-Myb-derived DNA binding domain mutations was also analyzed. All the samples were compared to cells infected with a control adenovirus. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity.
Keywords: repeat
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| |
|
| Overall design |
2 replicates of each type were analyzed. Replicates were performed independently, more than a week apart.
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| |
|
| Contributor(s) |
Liu F, Lei W, O'Rourke JP, Ness SA |
| Citation(s) |
16205643 |
| |
| Submission date |
Jun 14, 2005 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Scott A. Ness |
| E-mail(s) |
sness@salud.unm.edu
|
| Phone |
505-272-9883
|
| Organization name |
U. of New Mexico HSC
|
| Department |
Internal Medicine
|
| Lab |
Ness
|
| Street address |
MSC07 4025 - CRF 121
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87131-0001 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (8)
|
| GSM60915 |
control virus-infected replicate 1 (MCF7) |
| GSM60916 |
control virus-infected replicate 2 (MCF7) |
| GSM60917 |
cMyb virus-infected replicate 1 (MCF7) |
| GSM60918 |
cMyb virus-infected replicate 2 (MCF7) |
| GSM60919 |
3Mutc virus-infected replicate 1 (MCF7) |
| GSM60920 |
3Mutc virus-infected replicate 2 (MCF7) |
| GSM60921 |
vMyb virus-infected replicate 1 (MCF7) |
| GSM60922 |
vMyb virus-infected replicate 2 (MCF7) |
|
| Relations |
| BioProject |
PRJNA92417 |
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