|
| Status |
Public on Apr 08, 2011 |
| Title |
Expression data from control and LRF-deficient mouse germinal center B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
B cells are indispensable for humoral immunity, as they ultimately give rise to antibody-secreting plasma cells. During T cell-dependent antibody responses, naive B cells form germinal centers (GCs), a distinct histologic structure found in secondary lymphoid organs. Naive B cells become activated upon interaction with T cells and antigen presenting cells, and begin to rapidly proliferate and form the characteristic GC structure.
To elucidate the overall effect of LRF loss in the GCB cell transcriptome, gene expression microarray analysis of FACS-sorted GCB cells was performed. LRF Flox/+ mb-1 Cre+ mice were used as a control to normalize the potential effects of Cre recombinase, and four RNA samples for each genotype were used for the analysis.
|
| |
|
| Overall design |
B cell-specific LRF knockout (LRF Flox/Flox mb-1 Cre+) and control (LRF Flox/+ mb-1 Cre+) mice (4 each) were immunized with sheep red blood cells, and GCB cells were FACS-sorted 7 days later.
|
| |
|
| Contributor(s) |
Sakurai N, Li M, Takahiro M |
| Citation(s) |
21646720 |
| Submission date |
Apr 07, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Takahiro Maeda |
| Organization name |
Beckman Research Institute of the City of Hope
|
| Department |
Hematopoietic Stem Cell and Leukemia Research
|
| Lab |
Maeda
|
| Street address |
1500E Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA139145 |
Less...
More...