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Series GSE28613 Query DataSets for GSE28613
Status Public on Nov 22, 2011
Title MEDIATOR subunit med8 binding in Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Methylation profiling by genome tiling array
Summary The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silenced locations. We provide evidence that Mediator localization is guided by an interaction with histone tails, and that this interaction is regulated by their post-translational modifications. A quantitative, high-density genetic interaction map revealed links between Mediator components and factors affecting chromatin structure, especially histone deacetylases. Peptide binding assays demonstrated that pure wild type Mediator forms stable complexes with the tails of Histone H3 and H4. These binding assays also showed Mediator – histone H4 peptide interactions are specifically inhibited by acetylation of the histone H4 lysine 16, a residue critical in transcriptional silencing. Finally, these findings were validated by tiling array analysis, that revealed a broad correlation between Mediator and nucleosome occupancy in vivo, but a negative correlation between Mediator and nucleosomes acetylated at histone H4 lysine 16. Our studies show that chromatin structure and the acetylation state of histones are intimately connected to Mediator localization.
Overall design Med8-TAP strain ChIPed with IgG beads vs. Input in Saccharomyces cerevisiae
Contributor(s) Zhu X, Gustafsson C
Citation(s) 21742760
Submission date Apr 14, 2011
Last update date Jan 09, 2016
Contact name Claes M Gustafsson
Organization name Gothenburg University
Department Department of Medical biochemistry and cell biology
Street address Medcinaregatan 9A
City Gothenburg
ZIP/Postal code 405 30
Country Sweden
Platforms (1)
GPL7250 [Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array
Samples (4)
GSM707981 TAP_med8_1
GSM707982 Input DNA_1
GSM707983 TAP_med8_2
BioProject PRJNA138751

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28613_RAW.tar 108.5 Mb (http)(custom) TAR (of CEL) 15.1 Mb (ftp)(http) BAR 15.4 Mb (ftp)(http) TXT
Processed data are available on Series record

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