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| Status |
Public on Jul 26, 2013 |
| Title |
Transcriptional and translational effect of the lack of translation initiation factor eIF(iso)4E |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
Other
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| Summary |
One of the most regulated steps of translation initiation is the recruitment of an mRNA by the translation machinery. In eukaryotes, this step is mediated by the 5´end cap-binding factor eIF4E bound to the bridge protein eIF4G and forming the eIF4F complex. In plants, different isoforms of eIF4E and eIF4G form the antigenically distinct eIF4F and eIF(iso)4F complexes proposed to mediate selective translation. Using a microarray analysis of polyribosome- and non-polyribosome-purified mRNAs from 15 day-old Arabidopsis thaliana wild type [WT] and eIF(iso)4E knockout mutant [AteIF(iso)4E-1] seedlings we found 79 transcripts shifted from polyribosomes toward non-polyribosomes, and 47 mRNAs with the opposite behavior in the mutant. The translationally decreased mRNAs were overrepresented in root-preferentially expressed genes and proteins from the endomembrane system, including several transporters such as the phosphate transporter PHOSPHATE1 (PHO1), Sucrose transporter 3 (SUC3), the ABC transporter-like with ATPase activity (MRP11) and five electron transporters, as well as signal transduction-, protein modification- and transcription-related proteins.
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| Overall design |
For transcriptional analysis used total RNA of AteIF(iso)4E-1 seedlings of 15 days old to known the changes on transcripts leves by the eIF(iso)4E absence, using as control Wt seedlings. The experiments were performed in duplicate, and swap analysis were done. For translational analysis, used non-polysomal and polysomal RNA of AteIF(iso)4E-1 seedlings of 15 days old in order to known the transcripts that are modified in their translational levels by the eIF(iso)4E absence, using as control non polysomal and polysomal RNA of Wt seddlings.
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| Contributor(s) |
Martínez-Silva AV, Dinkova TD |
| Citation(s) |
22363683 |
| |
| Submission date |
May 18, 2011 |
| Last update date |
Apr 19, 2017 |
| Contact name |
TZVETANKA DIMITROVA DINKOVA |
| Organization name |
UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO, FACULTAD DE QUIMICA
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| Department |
BIOCHEMESTRY
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| Lab |
L-103, CONJUNTO E
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| Street address |
CIRCUITO EXTERIOR, CIUDAD UNIVERSITARIA DELEGACION COYOACAN
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| City |
MEXICO CITY |
| State/province |
DISTRITO FEDERAL |
| ZIP/Postal code |
04510 |
| Country |
Mexico |
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| Platforms (1) |
| GPL3513 |
Galbraith Laboratory Operon Long Oligonucleotide Microarray version 3.0 (ATv304.02.Z) |
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| Samples (6)
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| GSM726758 |
Mutant AteIF(iso)4E-1 polysomes versus Wt polysomes rep 1 |
| GSM726759 |
Mutant AteIF(iso)4E-1 polysomes versus Wt polysomes rep 2 |
| GSM726760 |
Mutant AteIF(iso)4E-1 non-polysomes versus Wt non-polysomes rep 1 |
| GSM726761 |
Mutant AteIF(iso)4E-1 non-polysomes versus Wt non-polysomes rep 2 |
| GSM726762 |
Mutant AteIF(iso)4E-1 versus Wt rep 1 |
| GSM726763 |
Mutant AteIF(iso)4E-1 versus Wt rep 2 |
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| Relations |
| BioProject |
PRJNA140031 |
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