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| Status |
Public on May 28, 2011 |
| Title |
Expression data from highly purified MMTV-Neu Tumor Initiating Cells (TICs) and the non-TIC CD24- fraction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The cancer stem cell model maintains that tumors are organized in a hierarchy driven by tumor initiating cells (TICs), and that patient survival inversely correlates with TIC gene expression. Here we generated a prognostic signature for HER2+ breast cancer from TICs purified from MMTV-Her2/Neu mammary tumors. TICs from this model, identified as Lin-:CD24+:JAG1- at a frequency of 2-5% by serial and single cell transplantation assays, showed elevated expression of proliferation genes and low expression of differentiation genes (compared to non-TIC fraction CD24- of the same tumor).
We used microarrays to detect differentially expressed genes in the TIC fraction compared to the non-TIC fraction of the same tumor
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| Overall design |
Cells from each primary tumor were separated by FACS into TIC and non-TIC fractions and total RNA was extracted and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Liu J, Deng T, Egan SE, Zacksenhaus E |
| Citation(s) |
22460789 |
| Submission date |
May 27, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Jeff Liu |
| E-mail(s) |
jeff.liu@utoronto.ca
|
| Phone |
416-340-4800
|
| Organization name |
University Health Network
|
| Department |
Princess Margaret Cancer Center
|
| Lab |
Zadeh Lab
|
| Street address |
101 College Street, RM 4-601
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| GSM732615 |
MMTV-Neu N251 Tumor, CD24- fraction |
| GSM732616 |
MMTV-Neu N251 Tumor, TIC fraction |
| GSM732617 |
MMTV-Neu N260 Tumor, CD24- fraction |
| GSM732618 |
MMTV-Neu N260 Tumor, TIC fraction |
| GSM732619 |
MMTV-Neu N283 Tumor, CD24- fraction |
| GSM732620 |
MMTV-Neu N283 Tumor, TIC fraction |
| GSM732621 |
MMTV-Neu N222+229 Tumor, CD24- fraction |
| GSM732622 |
MMTV-Neu N222+229 Tumor, TIC fraction |
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| Relations |
| BioProject |
PRJNA141273 |
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