|
| Status |
Public on May 28, 2011 |
| Title |
MMTV-Neu N222+229 Tumor, TIC fraction |
| Sample type |
RNA |
| |
|
| Source name |
Fraction of the tumor with highly purified Tumor Initiating Cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: primary mammary tumor
strain: FvB
genotype: MMTV-Neu
tumor size: less than 1.0 cm diameter
|
| Treatment protocol |
Cells from each tumor (with the exception of N222 and N229, which were combined in a 1:1 ratio to obtain enough cells for the experiment) were FACS sorted into TIC and non-TIC/CD24- fraction.
|
| Growth protocol |
Five independent primary mammary tumors from MMTV-Neu model (N250, N261, N283, N222, N229) were harvested and tumor cells were dissociated mechanically into single cell suspension.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from the 8 samples using PicoPure RNA Isolation Kit (Arcturus)
|
| Label |
biotin
|
| Label protocol |
Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Scan protocol |
Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Description |
Gene expression data from fraction of the tumor with highly purified Tumor Initiating Cells
|
| Data processing |
Microarray Data were normalized using RMA method via Partek software and absolute expression values were obtained
|
| |
|
| Submission date |
May 27, 2011 |
| Last update date |
May 28, 2011 |
| Contact name |
Jeff Liu |
| E-mail(s) |
jeff.liu@utoronto.ca
|
| Phone |
416-340-4800
|
| Organization name |
University Health Network
|
| Department |
Princess Margaret Cancer Center
|
| Lab |
Zadeh Lab
|
| Street address |
101 College Street, RM 4-601
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29590 |
Expression data from highly purified MMTV-Neu Tumor Initiating Cells (TICs) and the non-TIC CD24- fraction |
|
