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Status |
Public on Apr 17, 2012 |
Title |
Dynamics of two oscillation phenotypes in S. cerevisiae [2hr] |
Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
Sample organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by array
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Summary |
Genetic and environmental factors influence the phenotype of an organism. Time is rarely considered when studying changes in cellular phenotype. Time-resolved microarray data revealed genome-wide transcriptional changes in cells oscillating with ~2 and ~4 h periods. We mapped the global patterns of transcriptional oscillations into a 3-dimensional map to represent different cellular phenotypes of oscillation period. This map shows the dynamic nature of transcripts through time and concentration space, and that they are ordered and coupled to each other. Although cells differed in oscillation periods, transcripts involved in certain processes were conserved in a deterministic way. This ordered timing of biological process may allow cells to grow energetically efficient. Decreased glucose levels in the media were found to increase the redox cycles of yeast strain CEN.PK113-7D. Glucose may have acted as signaling molecules for timing longer catabolic processes in the cell population. As oscillation period lengthened, the peak to trough ratio of transcripts increased and the percent of cells in the unbudded (G0/G1) phase of the cell cycle increased. Gene transcripts appear to be coordinated with metabolic functions and the cell cycle.
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Overall design |
High-resolution samples over time were collected for CEN.PK113-7D oscillating with 2 hour period.
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Contributor(s) |
Chin SL, Marcus I, Klevecz RR, Li CM |
Citation(s) |
- Chin SL, Marcus IM, Klevecz RR, Li CM. Dynamics of oscillatory phenotypes in Saccharomyces cerevisiae reveal a network of genome-wide transcriptional oscillators. FEBS J 2012 Mar;279(6):1119-30. PMID: 22289124
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Submission date |
Jun 17, 2011 |
Last update date |
Feb 21, 2017 |
Contact name |
shwe lynn chin |
E-mail(s) |
shwe2414@yahoo.com
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Organization name |
Beckman Research Institute
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Department |
Biology
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Street address |
1500 E Duarte Rd.
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City |
Duarte |
ZIP/Postal code |
91010 |
Country |
USA |
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Platforms (1) |
GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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Samples (32)
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GSM743945 |
CEN.PK113-7D, 2h oscillation period 10 h |
GSM743946 |
CEN.PK113-7D, 2h oscillation period 10.06667 h |
GSM743947 |
CEN.PK113-7D, 2h oscillation period 10.13333 h |
GSM743948 |
CEN.PK113-7D, 2h oscillation period 10.2 h |
GSM743949 |
CEN.PK113-7D, 2h oscillation period 10.26667 h |
GSM743950 |
CEN.PK113-7D, 2h oscillation period 10.33333 h |
GSM743951 |
CEN.PK113-7D, 2h oscillation period 10.4 h |
GSM743952 |
CEN.PK113-7D, 2h oscillation period 10.46667 h |
GSM743953 |
CEN.PK113-7D, 2h oscillation period 10.53333 h |
GSM743954 |
CEN.PK113-7D, 2h oscillation period 10.6 h |
GSM743955 |
CEN.PK113-7D, 2h oscillation period 10.66667 h |
GSM743956 |
CEN.PK113-7D, 2h oscillation period 10.73333 h |
GSM743957 |
CEN.PK113-7D, 2h oscillation period 10.8 h |
GSM743958 |
CEN.PK113-7D, 2h oscillation period 10.86667 h |
GSM743959 |
CEN.PK113-7D, 2h oscillation period 10.93333 h |
GSM743960 |
CEN.PK113-7D, 2h oscillation period 11 h |
GSM743961 |
CEN.PK113-7D, 2h oscillation period 11.06667 h |
GSM743962 |
CEN.PK113-7D, 2h oscillation period 11.13333 h |
GSM743963 |
CEN.PK113-7D, 2h oscillation period 11.2 h |
GSM743964 |
CEN.PK113-7D, 2h oscillation period 11.26667 h |
GSM743965 |
CEN.PK113-7D, 2h oscillation period 11.33333 h |
GSM743966 |
CEN.PK113-7D, 2h oscillation period 11.4 h |
GSM743967 |
CEN.PK113-7D, 2h oscillation period 11.46667 h |
GSM743968 |
CEN.PK113-7D, 2h oscillation period 11.53333 h |
GSM743969 |
CEN.PK113-7D, 2h oscillation period 11.6 h |
GSM743970 |
CEN.PK113-7D, 2h oscillation period 11.66667 h |
GSM743971 |
CEN.PK113-7D, 2h oscillation period 11.73333 h |
GSM743972 |
CEN.PK113-7D, 2h oscillation period 11.79999974 h |
GSM743973 |
CEN.PK113-7D, 2h oscillation period 11.86666639 h |
GSM743974 |
CEN.PK113-7D, 2h oscillation period 11.93333304 h |
GSM743975 |
CEN.PK113-7D, 2h oscillation period 11.99999969 h |
GSM743976 |
CEN.PK113-7D, 2h oscillation period 12.06666634 h |
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This SubSeries is part of SuperSeries: |
GSE30053 |
Dynamics of two oscillation phenotypes in S. cerevisiae reveal a network of genome-wide transcriptional and cell cycle oscillators. |
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Relations |
BioProject |
PRJNA155159 |
Supplementary file |
Size |
Download |
File type/resource |
GSE30051_RAW.tar |
34.8 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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