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Status |
Public on Jul 14, 2011 |
Title |
[E-MTAB-587] PCR_artifacts |
Organism |
Arabidopsis thaliana |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
WARNING: This library was yield low amount of material and it was over-amplified by PCR. This libraries are used study the robustness of several statitical methods against PCR artifacts. ChIP experiments were performed on Arabidopsis wildtype inflorescences using an antibody raised against a C-terminal peptide of SEP3.
ArrayExpress Release Date: 2011-04-27
Publication Title: ChIP-seq Analysis in R (CSAR): An R package for the statistical detection of protein-bound genomic regions Publication Author List: Jose M. Muino, Kerstin Kaufmann, Roeland C. H. J. van Ham, Gerco C. Angenent, and Pawel Krajewski
Person Roles: submitter Person Last Name: Muino Person First Name: Jose Person Mid Initials: M. Person Email: jose.muino@wur.nl Person Phone: 0317-481122 Person Address: Droevendaalsesteeg 1, P.O. Box 16, 6700 AA Wageningen, The Netherlands Person Affiliation: Plant Research International B.V.
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Overall design |
Experimental Design: development_or_differentiation_design Experimental Design: in_vivo_design Experimental Design: ChiP-seq Experimental Factor Name: CHIP-ANTIBODY Experimental Factor Name: PCR_amplification Experimental Factor Type: Chip-antibody Experimental Factor Type: PCR_amplification
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Citation(s) |
21554688 |
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Submission date |
Jul 12, 2011 |
Last update date |
May 15, 2019 |
Organization |
European Bioinformatics Institute |
E-mail(s) |
miamexpress@ebi.ac.uk
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Lab |
ArrayExpress
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Street address |
Wellcome Trust Genome Campus
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City |
Hinxton |
State/province |
Cambridgeshire |
ZIP/Postal code |
CB10 1SD |
Country |
United Kingdom |
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Platforms (1) |
GPL9062 |
Illumina Genome Analyzer (Arabidopsis thaliana) |
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Samples (3) |
GSM759580 |
[E-MTAB-587] Inflorescences-Low_amount_material-SEP3_IP-rep1 |
GSM759581 |
[E-MTAB-587] Inflorescences-Low_amount_material-SEP3_IP-rep2 |
GSM759582 |
[E-MTAB-587] Inflorescences-Low_amount_material-OverAmplified-SEP3_IP-rep1 |
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Relations |
SRA |
ERP000559 |
ArrayExpress |
E-MTAB-587 |
BioProject |
PRJNA144527 |