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Series GSE30716 Query DataSets for GSE30716
Status Public on May 02, 2012
Title A cross-species analysis of microRNAs in the developing avian face
Organisms Anas platyrhynchos; Gallus gallus; Coturnix japonica
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Avian beaks show extreme species-specific variability in morphology, though they develop from the same primordial structures. In both humans and birds, cranial neural crest cells are the primary source of mesenchyme for the frontonasal prominence; previous work has shown that these cells contain molecular information that regulate species-specific facial variation. To determine the molecular basis of avian craniofacial patterning, we have used Next-Generation sequencing to profile all 20-40nt microRNAs from micro-dissected cranial neural crest cells from the frontonasal prominence of three bird species (chickens, quails, and ducks). Samples for each species were isolated at two developmental stages, before (Hamilton Hamburger stage [HH] 20) and after (HH25) morphological distinctions between the species are evident.
Overall design Examination of microRNA expression in frontonasal neural crest cells of 3 bird species at two developmental stages. Includes some biological replicates and one technical replicate.
Contributor(s) Powder KE, Ku Y, Lovett M
Citation(s) 22523571
Submission date Jul 15, 2011
Last update date Sep 23, 2019
Contact name Kara Powder
Organization name Washington University in St Louis
Department Genetics
Lab Lovett
Street address 4566 Scott Ave
City Saint Louis
State/province MO
ZIP/Postal code 63108
Country USA
Platforms (3)
GPL13797 Illumina Genome Analyzer IIx (Gallus gallus)
GPL13931 Illumina Genome Analyzer IIx (Coturnix japonica)
GPL13932 Illumina Genome Analyzer IIx (Anas platyrhynchos)
Samples (11)
GSM762009 Chicken_HH20_BS1
GSM762010 Chicken_HH25_BS1
GSM762011 Chicken_HH20_BS2
SRA SRP008226
BioProject PRJNA144377

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30716_RAW.tar 50.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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