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Series GSE31103 Query DataSets for GSE31103
Status Public on Oct 01, 2011
Title Changes in gene expression during cold exposure in Drosophila montana and D. virilis
Organisms Drosophila virilis; Drosophila montana
Experiment type Expression profiling by array
Summary We utilized a candidate gene approach using custom microarray constructed for our study species Drosophila montana and D. virilis to identify genes with modulated expression patterns under low temperature conditions. The flies were exposed to four different treatments (+5°C for 6 days, 0°C for 1 hour, two periods of recovery after cold stress and a control treatment). The aim of the study was to identify potential cold-responsive genes and to investigate differences in gene expression between the species. Microarray analysis revealed altogether 31 out of 219 genes on the array to show expression changes during different stages of cold stress. Among the potential stress tolerance genes detected earlier in D. melanogaster, hsr-omega was upregulated in both species during cold acclimation, expression changes in other genes being treatment- and species specific. Our microarray study clearly showed that different stages of cold response elicit changes at least in genes involved in heat shock response, circadian rhythm and metabolism.
 
Overall design Cold- induced gene expression was investigated comparing four different treatments to the control treatment: 1) Cold acclimation: 14 days in control conditions, then 6 days at +5°C 2) Cold hardening: 20 days in control conditions, then1 hour at 0°C 3) 15-min recovery from chill coma: the 20-day-old flies were exposed to -6°C for 16 hours, after which they were let to recover for 15 minutes 4) 1-hour recovery from chill coma: the 20-day-old flies were exposed to -6°C for 16 hours, after which they were let to recover for 1 hour. In control treatment flies were kept for 20 days at 19°C. All the flies were 20-days old at the time of sample collection, and the light:dark cycle was 22 hours of light and 2 hours of dark. Because of the limited space on the array plate, the recovery samples were collected only for D. montana. All the samples were collected 5-6 hours after the lights had been turned on in the chamber and the flies were immediately immersed in liquid nitrogen, after which they were stored at -84ºC. Three pools of ten flies were collected from each treatment group.
 
Contributor(s) Vesala L, Salminen TS, Laiho A, Hoikkala A, Kankare M
Citation(s) 22122733
Submission date Aug 01, 2011
Last update date Mar 23, 2012
Contact name Laura Vesala
E-mail(s) laura.vesala@jyu.fi
Organization name University of Jyväskylä
Department Department of Biological and Environmental Science
Street address Survontie 9
City Jyväskylä
ZIP/Postal code 40100
Country Finland
 
Platforms (1)
GPL14008 University of of Jyvaskyla FIN D. montana and D. virilis microarray 219
Samples (24)
GSM770014 D. montana whole body 19C control rep1
GSM770015 D. montana whole body 19C control rep2
GSM770016 D. montana whole body 19C control rep3
Relations
BioProject PRJNA144695

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31103_RAW.tar 17.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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