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| Status |
Public on Apr 01, 2012 |
| Title |
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Expression profiling by array
|
| Summary |
Reversible protein phosphorylation is an important and ubiquitous protein modification in all living cells. We report that protein arginine phosphorylation plays a physiological significant role for the regulation of protein activity. We detected 121 arginine phospho-sites for 87 proteins in the Gram-positive model organism Bacillus subtilis in vivo. Moreover, we provide evidences that arginine phosphorylations are involved in the fine-tuned signal transduction of many critical cellular processes, such as protein degradation, motility, competence, stringent and stress response. Our results suggest that in B. subtilis the activity of a protein arginine phosphatase allows a fast regulation of protein activity by protein arginine kinases and that protein arginine phosphorylations play an important role as a reversible post-translational modification in bacteria.
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| Overall design |
Cells were grown under vigorous agitation at 37 °C in a defined medium (Stülke et al., 1993, J Gen Microbiol 139, 2041-2045). Samples were taken at OD500 0.4 and 1h upon entry into stationary phase.
Microarray hybridizations were performed with RNA from three biological replicates. The individual samples were labeled with Cy5; a reference pool containing equal amounts of RNA from all 10 samples was labeled with Cy3.
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| Contributor(s) |
Elsholz AK, Mäder U, Michalik S, Kürsad T, Hecker M, Gerth U |
| Citation(s) |
22517742 |
| |
| Submission date |
Aug 08, 2011 |
| Last update date |
Apr 06, 2016 |
| Contact name |
Ulrike Mäder |
| E-mail(s) |
ulrike.maeder@uni-greifswald.de
|
| Organization name |
University Medicine Greifswald
|
| Department |
Functional Genomics
|
| Street address |
F.-L.-Jahn-Str. 15A
|
| City |
Greifswald |
| ZIP/Postal code |
D-17489 |
| Country |
Germany |
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| Platforms (1) |
| GPL10901 |
Bacillus subtilis Custom Agilent 44k v2 |
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| Samples (18)
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| GSM774609 |
B. subtilis wild type_exponential growth_rep1 |
| GSM774610 |
B. subtilis wild type_exponential growth_rep2 |
| GSM774611 |
B. subtilis wild type_exponential growth_rep3 |
| GSM774612 |
B. subtilis wild type_stationary phase_rep1 |
| GSM774613 |
B. subtilis wild type_stationary phase_rep2 |
| GSM774614 |
B. subtilis wild type_stationary phase_rep3 |
| GSM774615 |
B. subtilis ywlE mutant_exponential growth_rep1 |
| GSM774616 |
B. subtilis ywlE mutant_exponential growth_rep2 |
| GSM774617 |
B. subtilis ywlE mutant_exponential growth_rep3 |
| GSM774618 |
B. subtilis ywlE mutant_stationary phase_rep1 |
| GSM774619 |
B. subtilis ywlE mutant_stationary phase_rep2 |
| GSM774620 |
B. subtilis ywlE mutant_stationary phase_rep3 |
| GSM774621 |
B. subtilis ywlE mcsB mutant_exponential growth_rep1 |
| GSM774622 |
B. subtilis ywlE mcsB mutant_exponential growth_rep2 |
| GSM774623 |
B. subtilis ywlE mcsB mutant_exponential growth_rep3 |
| GSM774624 |
B. subtilis ywlE mcsB mutant_stationary phase_rep1 |
| GSM774625 |
B. subtilis ywlE mcsB mutant_stationary phase_rep2 |
| GSM774626 |
B. subtilis ywlE mcsB mutant_stationary phase_rep3 |
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| Relations |
| BioProject |
PRJNA146059 |
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