|
| Status |
Public on Nov 28, 2011 |
| Title |
Gene expression profiles of Six1/2-1(RNAi) animals and POU2/3(RNAi) animals |
| Organism |
Schmidtea mediterranea |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this study was to identify genes that were expressed in the protonephridial system. Because Six1/2(RNAi) and POU2/3(RNAi) animals lose the tubules, flame cells and accessory cells, these animals were expected to show decreased levels of the genes expressed in the nephridial system relative to control RNAi animals.
|
| |
|
| Overall design |
RNA from Six1/2(RNAi) and POU2/3(RNAi) animals was harvested with Trizol at days 8 and 15 after RNAi initiation for the former and days 15 and 21 for the latter. Three biological replicates were used. RNA from the four samples were competetively hybridized on a microarray with RNA from control RNAi animals of the corresponding days.
|
| |
|
| Contributor(s) |
Scimone ML, Bell GW, Reddien PW |
| Citation(s) |
21937596 |
| |
| Submission date |
Aug 23, 2011 |
| Last update date |
Mar 05, 2013 |
| Contact name |
Mansi Srivastava |
| E-mail(s) |
mansi@wi.mit.edu
|
| Organization name |
Whitehead Institutue
|
| Lab |
Reddien
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14150 |
Agilent-021750 Schmidtea mediterranea 45K Smed_WI_expression_chip_Reddien_v2 015536 (Feature Number version) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA145527 |
Less...
More...