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Series GSE32196 Query DataSets for GSE32196
Status Public on Sep 18, 2011
Title nup42-delta cells pretreated with NaCl lose the altered gene expression in response to H2O2
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary BY4741 (S288c haploid) cells have different gene expression in response to 0.4mM H2O2 when pretreated with 0.7M NaCl compared to cells that are not pretreated with NaCl (mock cells). Cells lacking Nup42p do not exhibit a different gene expression in response to H2O2 between naive cells and cells pretreated with 0.7M NaCl
 
Overall design BY4741 and nup42-delta cells were independently grown in log phase for at least 7 doublings (T0) and then the genomic expression of "mock" cells to 0.4mM H2O2 was assessed every 10 mins for 20 mins. To study the effects of NaCl treatment on H2O2 response, cells were grown in log phase at least 7 doublings (T0) and then the genomic expression of cells was measured in response to 0.7M NaCl at 30 mins and the treatment continued for 60 minutes. The cells were then removed from stress and grown in stress free media for four hours (memory t0). Then these cells were exposed to 0.4mM H2O2 and the genomic expression was measured every 10 minutes during the H2O2 timecourse of 20 minutes.
 
Contributor(s) Qiaoning G, Haroon S, Hose J, Gasch AP
Citation(s) 22851651
Submission date Sep 17, 2011
Last update date Nov 06, 2012
Contact name Suraiya Ruma Haroon
E-mail(s) haroon@wisc.edu
Phone (608) 265-0863
Organization name University of Wisconsin - Madison
Department Genetics
Lab Gasch
Street address 425 Henry Mall
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (2)
GPL5915 UWAPG Yeast 6K v1.0
GPL15778 NimbleGen UW-Madison Saccharomyces cerevisiae 380K tiling array [2006-10-12_Ansari_tiling_51mer; gene-level version]
Samples (43)
GSM797774 BY4741 + 0.7M NaCl 15 min vs 0 min
GSM797775 BY4741 + 0.7M NaCl 30 min vs 0 min rep1
GSM797776 BY4741 + 0.7M NaCl 30 min vs 0 min rep2
Relations
BioProject PRJNA147377

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32196_RAW.tar 127.3 Mb (http)(custom) TAR (of FTR, GPR)
Processed data included within Sample table
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