|
| Status |
Public on Dec 31, 2011 |
| Title |
Effects of Plasmodium berghei infection on Anopheles gambiae during oocysts development |
| Organism |
Anopheles gambiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Plasmodium ssp. are pathogens in their vertebrate hosts and also cause deleterious effects to their insect vectors. We show here, however, that Plasmodium-infected mosquitoes more efficiently accumulate energy resources (glycogen) during oocyst development, and survive better when they are starved. Microarray analysis revealed that mosquito metabolism is altered by the presence of rapidly growing oocysts in the midgut. Plasmodium infection is associated with enhanced expression of several insulin-like peptides in mosquitoes and blocking insulin-like signaling results in diminished Plasmodium development. We conclude that Plasmodium infection dramatically changes mosquito metabolism pathways, epitomized by enhanced insulin-signaling, and thereby confer a survival advantage to the insects during periods of starvation. Manipulation of this pathway may provide new strategies to influence the ability of mosquitoes to transmit the protozoa that cause malaria.
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| Overall design |
One-condition experiment: P. berghei infected vs uninfected mosquitoes; 10 mosquitoes per sample; 4 biological replicates for each group; two different time points, 10 and 17 days post infection; control mosquitoes were from same batch as infected mosquitoes that were fed on clean mice.
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| |
|
| Contributor(s) |
Zhao YO, Kurscheid S |
| Citation(s) |
22808193 |
| Submission date |
Sep 18, 2011 |
| Last update date |
Dec 18, 2012 |
| Contact name |
Sebastian Kurscheid |
| E-mail(s) |
sebastian.kurscheid@anu.edu.au
|
| Organization name |
Australian National University
|
| Department |
JCSMR
|
| Lab |
Prof David Tremethick
|
| Street address |
57 Garran Road
|
| City |
Acton |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL13157 |
Agilent-020449 Mosquito Gene Expression Microarray (Probe name version) |
|
| Samples (8)
|
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| Relations |
| BioProject |
PRJNA147385 |
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