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| Status |
Public on Aug 03, 2012 |
| Title |
IBM1, a JmjC domain histone demethylase, is involved in the regulation of RNA-directed DNA methylation through epigenetic control of RDR2 and DCL3 expression in Arabidopsis. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The small RNA mediated DNA methylation (RdDM) is highly elaborated in Arabidopsis as an important epigenetic pathway controls expression of genes and multiple developmental processes. RDR2 and DCL3 are necessary factors to the 24-nt siRNA biogenesis and the RdDM pathway. Here, we find that IBM1 (increase in BONSAI methylation 1), a conserved JmjC family histone demethylase, could directly associated with the chromatin of RDR2 and DCL3. The IBM1 mutation induces hypermethylation on both H3K9 and DNA non-CG sites in these loci, and further represses their expression. The reduction of RDR2 and DCL3 may affect siRNA biogenesis in a locus-specific manner and disrupt the RdDM directed repression. It suggests an indirect way to regulate targets by IBM1 through control of siRNA biogenesis, and demonstrate the relationship between histone methylation and small RNAs.
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| Overall design |
Genome-wide integrated mapping of mRNA transcripts and small RNAs, using whole plant of 10-days seedlings of the Columbia-0 wildtype and ibm1-3 mutant Arabidopsis thaliana.
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| Contributor(s) |
Fan D, Wang X, Zhang J, Ma L |
| Citation(s) |
22772985 |
| |
| Submission date |
Sep 21, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Di Fan |
| E-mail(s) |
scwwxc@163.com
|
| Organization name |
National Institute of Biological Sciences
|
| Street address |
7# Science Park Road Zhongguancun Life Science Park
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
10085 |
| Country |
China |
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| Platforms (1) |
| GPL9062 |
Illumina Genome Analyzer (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| SRA |
SRP008486 |
| BioProject |
PRJNA146353 |
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