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Series GSE32294 Query DataSets for GSE32294
Status Public on Apr 04, 2012
Title Expression regulation by RYBP in mouse embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Genome binding/occupancy profiling by array
Summary Polycomb repressive complexes (PRCs) are important chromatin regulators of ES cell function. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B, and has been suggested to participate in localizing Polycomb complexes to their targets. Moreover, constitutive inactivation of RYBP precludes ES cell formation. Here we have used ES cells conditionally deficient in RYBP to investigate RYBP function. Chromosome immunoprecipitation on a chip (ChIP-chip) of RYBP and microarray experiments were performed using wild type and knocked-out ES cells.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Gene expression profiling of WT, conditionally deficient in RYBP with or without Yaf2 RNAi, and ChIP-chip of RYBP on promoters of WT, Dnmt1-KO or Eed-KO ES cells.
 
Contributor(s) Hisada K, Endoh M, Endo TA, Vidal M
Citation(s) 22269950
Submission date Sep 21, 2011
Last update date Feb 11, 2019
Contact name Takaho A. Endo
E-mail(s) takaho.endo@riken.jp
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
 
Platforms (3)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL14573 Agilent-014716 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 (G4490A) (Probe Name version)
GPL14597 Agilent-014717 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 (G4490A) (Probe Name version)
Samples (28)
GSM799870 Rybp#10 (OHT-)_4d_rep1
GSM799871 Rybp#10 (OHT+)_4d_rep1
GSM799872 Rybp#15 (OHT-)_4d_rep1
This SuperSeries is composed of the following SubSeries:
GSE32287 Expression data from wild-type and Rybp-deficient ES cells
GSE32288 Gene expression profile in Yaf2 KD and/or RYBP KO ES cells.
GSE32289 Rybp ChIP-chip in mouse ES cells
Relations
BioProject PRJNA146367

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32294_RAW.tar 519.8 Mb (http)(custom) TAR (of CEL, TXT)

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