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Status |
Public on Apr 04, 2012 |
Title |
Expression regulation by RYBP in mouse embryonic stem cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array Genome binding/occupancy profiling by array
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Summary |
Polycomb repressive complexes (PRCs) are important chromatin regulators of ES cell function. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B, and has been suggested to participate in localizing Polycomb complexes to their targets. Moreover, constitutive inactivation of RYBP precludes ES cell formation. Here we have used ES cells conditionally deficient in RYBP to investigate RYBP function. Chromosome immunoprecipitation on a chip (ChIP-chip) of RYBP and microarray experiments were performed using wild type and knocked-out ES cells.
This SuperSeries is composed of the SubSeries listed below.
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Overall design |
Gene expression profiling of WT, conditionally deficient in RYBP with or without Yaf2 RNAi, and ChIP-chip of RYBP on promoters of WT, Dnmt1-KO or Eed-KO ES cells.
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Contributor(s) |
Hisada K, Endoh M, Endo TA, Vidal M |
Citation(s) |
22269950 |
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Submission date |
Sep 21, 2011 |
Last update date |
Feb 11, 2019 |
Contact name |
Takaho A. Endo |
E-mail(s) |
takaho.endo@riken.jp
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Organization name |
RIKEN
|
Department |
IMS
|
Lab |
Laboratory for Integrative Genomics
|
Street address |
1-7-22 Suehiro, Tsurumi
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City |
Yokohama |
State/province |
Kanagawa |
ZIP/Postal code |
230-0045 |
Country |
Japan |
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Platforms (3) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
GPL14573 |
Agilent-014716 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 (G4490A) (Probe Name version) |
GPL14597 |
Agilent-014717 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 (G4490A) (Probe Name version) |
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Samples (28)
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This SuperSeries is composed of the following SubSeries: |
GSE32287 |
Expression data from wild-type and Rybp-deficient ES cells |
GSE32288 |
Gene expression profile in Yaf2 KD and/or RYBP KO ES cells. |
GSE32289 |
Rybp ChIP-chip in mouse ES cells |
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Relations |
BioProject |
PRJNA146367 |