|
| Status |
Public on Oct 06, 2011 |
| Title |
Secretoneurin: A new hormone? Auratus: The goldfish environmental genomics project |
| Platform organisms |
Carassius auratus; Cyprinus carpio |
| Sample organism |
Carassius auratus |
| Experiment type |
Expression profiling by array
|
| Summary |
The objectives of this study were to examine the effect of secretoneurin (SN) on pituitary gene expression. A mixture of goldfish pituitaries from both males and females were dispersed using Typsin type II and DNase II enzymes. The cells were plated in 24-well culture plates at a density of ~2.5 x 105 cells/well. After at least 12-hr plating period, cells were washed with testing medium and pre-incubated for 1 hr to stabilize basal LH secretion. Dispersed pituitary cells were treated with 10 nM gfSN for 12 hours in static incubation after which the cells were carefully washed by 1X PBS. Total RNA was isolated by utilizing RNeasy Micro Kit (QIAGEN GmbH, Hilden, Germany) following the company’s standard protocol. RNA concentrations were determined using the NanoDrop ND-100 spectrophotometer (Thermo Scientific, Wilmington, DE). cDNA synthesis, cDNA purification and microarray slide hybridization followed our validated and established microarray protocol (Martyniuk et al., 2006; Zhang et al., 2009; Popesku et al., 2010).
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| |
|
| Overall design |
4 control and 4 treatment samples were hybridized using dual channel micrarray with cy3 and cy5
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| |
|
| Contributor(s) |
Basak A, Decatur W, Hu H, Martyniuk C, Volkoff H, Zhao E, Trudeau V |
| Citation(s) |
22036841 |
| |
| Submission date |
Oct 05, 2011 |
| Last update date |
Aug 06, 2013 |
| Contact name |
Christopher Joseph Martyniuk |
| E-mail(s) |
CMARTYN@UFL.EDU
|
| Organization name |
UF
|
| Department |
Physiological Sciences
|
| Street address |
CEHT Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3735 |
AURATUS: The goldfish environmental genomics project v1.0 |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA147129 |
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