|
| Status |
Public on Oct 06, 2011 |
| Title |
gf Pituitary cells_3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
pituitary cells with SN incubation
|
| Organism |
Carassius auratus |
| Characteristics |
tissue: male and female pituitaries
treatment: with SN incubation
|
| Treatment protocol |
Dispersed pituitary cells were treated with 10 nM gfSN for 12 hours in static incubation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the TRIzol reagent (Invitrogen, Burlington, ON, Canada) per the manufacturer's protocol. Samples were treated with DNase on-column in an RNeasy Mini Plus kit (Qiagen, Mississauga, ON, Canada). RNA quantity was evaluated using the NanoDrop. RNA integrity was evaluated using the BioAnalyzer (Agilent);
|
| Label |
Cy5
|
| Label protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
| |
|
| Channel 2 |
| Source name |
pituitary cells with no incubation
|
| Organism |
Carassius auratus |
| Characteristics |
tissue: male and female pituitaries
treatment: with no incubation
|
| Treatment protocol |
Dispersed pituitary cells were treated with 10 nM gfSN for 12 hours in static incubation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the TRIzol reagent (Invitrogen, Burlington, ON, Canada) per the manufacturer's protocol. Samples were treated with DNase on-column in an RNeasy Mini Plus kit (Qiagen, Mississauga, ON, Canada). RNA quantity was evaluated using the NanoDrop. RNA integrity was evaluated using the BioAnalyzer (Agilent);
|
| Label |
Cy3
|
| Label protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
| |
|
| |
| Hybridization protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
| Scan protocol |
Microarrays were scanned at full speed 10 micron resolution using the ScanArray 5000 XL system (Packard Biosciences/PerkinElmer) using both red and blue lasers. Images were obtained with ScanArray Express software using automatic calibration sensitivity varying PMT gain (PMT starting at 65% for Cy5 and 70% for Cy3) with fixed laser power at 80% and the target intensity set for 90%.
|
| Description |
slide 43
|
| Data processing |
Microarray images were analyzed using QuantArray (Packard Biosciences/PerkinElmer) and raw signal intensity values were obtained for duplicate spots of genes. Normalization was performed using LOWESS
|
| |
|
| Submission date |
Oct 05, 2011 |
| Last update date |
Oct 06, 2011 |
| Contact name |
Christopher Joseph Martyniuk |
| E-mail(s) |
CMARTYN@UFL.EDU
|
| Organization name |
UF
|
| Department |
Physiological Sciences
|
| Street address |
CEHT Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
| |
|
| Platform ID |
GPL3735 |
| Series (1) |
| GSE32629 |
Secretoneurin: A new hormone? Auratus: The goldfish environmental genomics project |
|
