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| Status |
Public on Oct 08, 2011 |
| Title |
Integrative Survival-Based Molecular Profiling of Human Pancreatic Cancer [miRNA] |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
To perform an integrative profile of human pancreatic cancer (PDAC) to identify prognosis-significant genes and their related pathways.
A concordant survival-based whole genome in silico array analysis of DNA copy number, and mRNA & micro RNA (miRNA) expression in 25 early stage PDAC was performed. A novel composite score simultaneously integrated gene expression with regulatory mechanisms to identify the signature genes with the most levels of prognosis-significant evidence. The predominant signaling pathways were determined via a pathway-based approach. Independent patient cohorts (n= 150 and 42) were then used as in vitro validation of the array findings. We find that EGFR, SRC signaling, and PI3K/AKT pathway activation are strongly linked to clinical disease progression. Furthermore, we identify two discrete subsets of pancreatic tumors characterized by either SRC or PI3K/AKT signaling that may dictate variable responses to targeted therapy.
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| Overall design |
42 human PDAC tumors and 7 non-malignant pancreas samples snap-frozen at the time of surgery were chosen. Representative H&E sections of each were evaluated by a practicing gastrointestinal pathologist to confirm diagnosis and determine relative percentage of malignant cells. Samples with tumor cell content >30% were chosen for final multi-platform analysis (N=25). Genomic miRNAa of human pancreatic samples were profiled on miRCURY LNA™ microRNA Array kit v.11.0 - human, mouse & rat arrays per manufacturer's instructions.
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| Contributor(s) |
Donahue TR, Tran LM, Hill R, Li Y, Kovochich A, Calvopina JH, Patel SG, Wu N, Hindoyan A, Farrell JJ, Li X, Dawson DW, Wu H |
| Citation(s) |
22261810, 25846727 |
| |
| Submission date |
Oct 06, 2011 |
| Last update date |
Apr 16, 2020 |
| Contact name |
Linh My Tran |
| E-mail(s) |
linhtran@ucla.edu
|
| Organization name |
University of Los Angeles
|
| Department |
Medicine - Pulmonary & Critical Care
|
| Lab |
Dubinett Lab
|
| Street address |
Box 951690, 37-131 CHS
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095-1690 |
| Country |
USA |
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| Platforms (1) |
| GPL7723 |
miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno |
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| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE32688 |
Integrative Survival-Based Molecular Profiling of Human Pancreatic Cancer |
|
| Relations |
| BioProject |
PRJNA154381 |
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