|
| Status |
Public on Aug 22, 2013 |
| Title |
Transcriptome analysis of Bacillus subtilis in response to air plasma treatment |
| Platform organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Sample organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we present a follow up investigation on the effects of argon plasma on vegetative growing Bacillus subtilis 168 (see GSE27113). A growth chamber system suitable for low temperature gas plasma treatment of bacteria in liquid medium enabled a first glimpse on the
complex cellular response. In order to gain further knowledge, a second kind of plasma treatment was applied and combined proteomic and transcriptomic analyses were used to investigate the specific stress response of B. subtilis cells to treatment with not only argon but also
air plasma. Besides similar cellular responses, due to both argon and air plasma treatment, a variety of different responses such as growth retardation and morphological changes were observed. Furthermore, we analyzed the specific response to DNA damages and oxidative stress.
The biological findings such as oxidative stress responses were supported by the detection of reactive plasma species by OES and FTIR measurements.
|
| |
|
| Overall design |
Cells were grown in LB medium. At OD540 of 0.5, air plasma treatment was set for 15 min with a discharge power of 7.4 W. Samples for mircroarray analysis were taken 5 min after the 15 min plasma treatment.
Microarray hybridizations were performed with RNA from three biological replicates. The individual samples were labeled with Cy5; a reference pool containing equal amounts of RNA from all samples was labeled with Cy3.
|
| |
|
| Contributor(s) |
Winter T, Bernhardt J, Mäder U, Winter J, Sietmann R, Weltmann K, Hecker M, Kusch H |
| Citation(s) |
23794223 |
| |
| Submission date |
Oct 11, 2011 |
| Last update date |
Apr 06, 2016 |
| Contact name |
Ulrike Mäder |
| E-mail(s) |
ulrike.maeder@uni-greifswald.de
|
| Organization name |
University Medicine Greifswald
|
| Department |
Functional Genomics
|
| Street address |
F.-L.-Jahn-Str. 15A
|
| City |
Greifswald |
| ZIP/Postal code |
D-17489 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL10901 |
Bacillus subtilis Custom Agilent 44k v2 |
|
| Samples (6)
|
| GSM813530 |
B. subtilis_air plasma treated_replicate2 |
| GSM813531 |
B. subtilis_control_replicate3 |
| GSM813532 |
B. subtilis_air plasma treated_replicate3 |
|
| Relations |
| BioProject |
PRJNA146551 |
Less...
More...