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| Status |
Public on Mar 24, 2014 |
| Title |
Using tiling arrays to precisely detect DNA targets of THO in yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by array
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Crosslinked DNA from wild type cells or from mutant of the THO complex was analyzed by tiling arrays to precisely detect DNA targets of THO in yeast.
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| Overall design |
DNA coming from SDS-washed DCF pellet and the supernatant SN2k of 2 strains were compared in the study, WT (W303) and mft1 delta (DLY224). The DNA of each condition (Pellet or Supernatant) was labeled either with Cy3 or Cy5 fluorescent dyes and competitively hybridized on 244k agilent arrays. For each strain, the experiment was performed on 2 biological replicates, with dye swap.
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| Contributor(s) |
Mouaikel J, Causse S, Daubenton-Carafa Y, Blugeon C, Lemoine S, Devaux F, Rougemaille M, Darzac X, Libri D |
| Citation(s) |
24210826 |
| |
| Submission date |
Oct 14, 2011 |
| Last update date |
Feb 05, 2015 |
| Contact name |
sophie lemoine |
| E-mail(s) |
slemoine@biologie.ens.fr
|
| Organization name |
Ecole Normale Supérieure
|
| Department |
Biologie
|
| Lab |
Plateforme transcriptome
|
| Street address |
46 rue d'Ulm
|
| City |
PARIS |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL4130 |
Agilent-014741 Yeast Whole Genome ChIP-on-Chip Microarray 244K (G4491A) |
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| Samples (4)
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| GSM816938 |
WT pellet vs WT supernatant (1 of 2) |
| GSM816939 |
WT pellet vs WT supernatant (2 of 2) |
| GSM816940 |
mft1 delta pellet vs mft1 delta supernatant (1 of 2) |
| GSM816941 |
mft1 delta pellet vs mft1 delta supernatant (2 of 2) |
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| Relations |
| BioProject |
PRJNA146431 |
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