|
| Status |
Public on May 01, 2012 |
| Title |
A major genome region underlying artemisinin resistance in malaria |
| Organism |
Plasmodium falciparum |
| Experiment type |
Genome variation profiling by genome tiling array
|
| Summary |
Evolving resistance to artemisinin-based compounds in SE Asia threatens to derail attempts to control and eliminate malaria. Resistance has been confirmed in western Cambodia, has recently emerged in western Thailand, but is absent from neighboring Laos. Artemisinin resistance results in reduced parasite clearance rates (CR) from the blood following treatment. We used a two-phase approach to identify the genes underlying this ongoing selective event. Comparison of geographical differentiation and haplotype structure at 6,969 polymorphic SNPs in 91 parasites from western Cambodia, western Thailand and Laos identified 33 strongly selected genome regions. We screened SNPs and microsatellites within these genome regions in 718 parasites from western Thailand, and identified a 35kb region of chr 13 showing strong association (P=10-6 to 10-11) with slow CR. This region contains several compelling candidate loci, such as HSP70, for assessment by transfection. These results illustrate the efficacy of targeted association for identifying the genetic basis of adaptive traits.
|
| |
|
| Overall design |
91 malaria parasite isolates assayed for single nucleotide polymorphisms across 45K loci
|
| |
|
| Contributor(s) |
Cheeseman IH, Anderson TJ |
| Citation(s) |
22491853 |
| Submission date |
Oct 14, 2011 |
| Last update date |
Feb 18, 2014 |
| Contact name |
Michael Ferdig |
| E-mail(s) |
ferdig.1@nd.edu
|
| Phone |
(574)631-9973
|
| Organization name |
University of Notre Dame
|
| Department |
Biological Sciences
|
| Lab |
Ferdig Lab
|
| Street address |
280 Galvin Life Science
|
| City |
Notre Dame |
| State/province |
IN |
| ZIP/Postal code |
46556 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (167)
|
|
| Relations |
| BioProject |
PRJNA147999 |
Less...
More...