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| Status |
Public on Oct 25, 2012 |
| Title |
Expression data from WT DN3, TCF-1-deficient DN3 thymocytes, and T cell lymphomas in TCF-1-deficient animals. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
TCF-1 is an HMG family transcription factor which is known to be critical for T cell development. We discovered that it has a unique role in suppressing malignant transformation of developing thymocytes at early stages. We identified ID2 and LEF-1 as key TCF-1 target genens in tumor suppression.
We used microarrays to detect gene expression changes in WT and TCF-1 deficient DN3 thymocytes as well as T cell lymphoma cells developed in TCF-1 KO mice.
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| Overall design |
DN3 thymocytes were directly sorted from WT or TCF-1 KO mice. T cell lymphoma blast cells were also sorted from TCF-1 KO mice that developed the disease. RNA was extracted and hybridized to GeneChip Mouse GENE 1.0 ST arrays (Affymetrix).
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| |
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| Contributor(s) |
Xue H, Yu S |
| Citation(s) |
23103132 |
| |
| Submission date |
Oct 27, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Hai-Hui Xue |
| E-mail(s) |
haihui.xue@hmh-cdi.org
|
| Organization name |
Hackensack University Medical Center
|
| Department |
Center for Discovery and Innovation
|
| Street address |
111 Ideation Way
|
| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (12)
|
| GSM823502 |
WT DN3 thymocytes, biological rep 1 |
| GSM823503 |
WT DN3 thymocytes, biological rep 2 |
| GSM823504 |
WT DN3 thymocytes, biological rep 3 |
| GSM823505 |
TCF-1 KO DN3 thymocytes, biological rep 1 |
| GSM823506 |
TCF-1 KO DN3 thymocytes, biological rep 2 |
| GSM823507 |
TCF-1 KO DN3 thymocytes, biological rep 3 |
| GSM823508 |
T cell lymphomas in TCF-1 KO mice, biological rep 1 |
| GSM823509 |
T cell lymphomas in TCF-1 KO mice, biological rep 2 |
| GSM823510 |
T cell lymphomas in TCF-1 KO mice, biological rep 3 |
| GSM823511 |
T cell lymphomas in TCF-1 KO mice, biological rep 4 |
| GSM823512 |
T cell lymphomas in TCF-1 KO mice, biological rep 5 |
| GSM823513 |
T cell lymphomas in TCF-1 KO mice, biological rep 6 |
|
| Relations |
| BioProject |
PRJNA149283 |
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