|
| Status |
Public on Oct 25, 2012 |
| Title |
WT DN3 thymocytes, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse WT DN3 thymocytes
|
| Organism |
Mus musculus |
| Characteristics |
tissue: thymus
cell type: thymocytes
strain: C57/BL6
genotype: wild-type
age: 8-12 wks
|
| Treatment protocol |
DN3 thymocytes or T cell lymphoma cells were sorted directly into Trizol LS reagent.
|
| Growth protocol |
DN3 thymocytes were isolated by cell sorting from WT or TCF-1-deficient mice. T cell lymphomas were sorted from the thymuses of TCF-1 KO mice that have developed the diseases.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by further purification on RNeasy columns.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared using NuGEN WT-Ovation PicoRNA amplification system, starting from 800 pg to 50 ng of total RNA.
|
| |
|
| Hybridization protocol |
2.5 ug of labeld cDNA were hybridized on mouse GENE 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner 3000 complete with a 7G upgrade.
|
| Description |
Gene expression data from WT DN3 thymocytes
|
| Data processing |
The data were analyzed with Partek GS v 6.4 using default RMA as normalization method.
probe group file: MoGene-1_0-v1.r4.pgf
meta-probeset file: MoGene-1_0-v1.r4.mps
|
| |
|
| Submission date |
Oct 27, 2011 |
| Last update date |
Oct 25, 2012 |
| Contact name |
Hai-Hui Xue |
| E-mail(s) |
haihui.xue@hmh-cdi.org
|
| Organization name |
Hackensack University Medical Center
|
| Department |
Center for Discovery and Innovation
|
| Street address |
111 Ideation Way
|
| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE33292 |
Expression data from WT DN3, TCF-1-deficient DN3 thymocytes, and T cell lymphomas in TCF-1-deficient animals. |
|
