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| Status |
Public on Aug 30, 2012 |
| Title |
CcpA, a Pleiotropic Key Regulator in Butanol-producing Clostridium acetobutylicum ATCC 824 |
| Organism |
Clostridium acetobutylicum |
| Experiment type |
Expression profiling by array
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| Summary |
Clostridium acetobutylicum is a typical bacterium of major importance to industrial butanol production. In order to dissect the regulatory network pertaining to the industrial application of this bacterium, catabolite control protein A (CcpA) was investigated for its global function by DNA microarray.It showed that CcpA of C. acetobutylicum controls hundreds of genes, not only carbon metabolism, but also solvent production and sporulation in the life cycle.The results here demonstrated that CcpA is an important pleiotropic regulator related to some specific physiological and biochemical process in butanol-producing C. acetobutylicum.
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| Overall design |
In order to enable a global understanding of the regulatory roles of CcpA when fermenting mixed sugars, which is of great significance in utilization of lignocellulosic hydrolysates, D-glucose plus D-xylose were used as the carbon sources in fermentation for microarray analysis. Microarray analysis was performed at four time points:the time point M and L were chosen both in acidogenic phase, while the time point T and S were chosen in shift phase (from acidogenesis to solventogenesis) and solventogenic phase, respectively.One-color microarray assays were performed.Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US). The ratio of transcript level between wildtype and mutant can been achieved using the formula: 2^(value of wildtype)/2^(value of ccpA mutant).
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| Contributor(s) |
Ren C, Gu Y, Hu S, Wu Y, Yang S, Jiang W |
| Citation(s) |
22846451 |
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| Submission date |
Nov 01, 2011 |
| Last update date |
Aug 30, 2012 |
| Contact name |
Cong Ren |
| E-mail(s) |
rencong@sibs.ac.cn
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| Organization name |
Institute of Plant Physiology and Ecology, SIBS, CAS.
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| Street address |
300 Feng Lin Road
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL14821 |
Jiang lab Clostridium acetobutylicum ATCC 824 agilent 8×15K one-color v1 |
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| Samples (8)
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| GSM825160 |
clostridium acetobutylicum ATCC 824 middle exponential phase (M) |
| GSM825161 |
clostridium acetobutylicum ATCC 824 late exponential phase (L) |
| GSM825162 |
clostridium acetobutylicum ATCC 824 transition phase (T) |
| GSM825163 |
clostridium acetobutylicum ATCC 824 stationary phase (S) |
| GSM825164 |
clostridium acetobutylicum ATCC 824 ccpA mutant middle exponential phase (M) |
| GSM825165 |
clostridium acetobutylicum ATCC 824 ccpA mutant late exponential phase (L) |
| GSM825166 |
clostridium acetobutylicum ATCC 824 ccpA mutant transition phase (T) |
| GSM825167 |
clostridium acetobutylicum ATCC 824 ccpA mutant stationary phase (S) |
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| Relations |
| BioProject |
PRJNA148997 |
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