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| Status |
Public on Aug 01, 2012 |
| Title |
Sugarcane Expression Data from Stress Time Series |
| Platform organism |
Saccharum officinarum |
| Sample organism |
Saccharum hybrid cultivar (mixed) |
| Experiment type |
Expression profiling by array
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| Summary |
Sugarcane one-eyed seed sets obtained from a variety sensitive to drought conditions (cv. SP90-1638, CTC, Brazil) were cultivated on moist sand for 15 days. The plants were transferred to pots containing moist sand and were irrigated with Hoagland's solution (Hoagland and Arnon, 1950). Regular watering was maintained for 90 days and suppressed after this period for the experimental group. Leaves were collected 24 h, 72 h and 120 h after exposure to drought conditions for the control and experimental groups. Samples were collected and immediately frozen in liquid nitrogen. Leaves from six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response
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| Overall design |
RNA from experimental sample and from its respective control sample (untreated plants) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental point. The quantification of each hybridization was submitted in two files, one for each slide side (technical replicates).
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| Contributor(s) |
Lembke CG, Nishiyama MY Jr, Sato PM, de Andrade RF, Souza GM |
| Citation(s) |
22610347 |
| |
| Submission date |
Nov 09, 2011 |
| Last update date |
Aug 03, 2016 |
| Contact name |
Milton Yutaka Nishiyama Jr |
| E-mail(s) |
yutakajr@gmail.com
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| URL |
http://sucest-fun.org
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| Organization name |
Universidade de São Paulo - USP
|
| Department |
Biochemistry
|
| Lab |
Signal Transduction Laboratory
|
| Street address |
Av. Professor Lineu Prestes, 748
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| City |
São Paulo |
| State/province |
São Paulo |
| ZIP/Postal code |
05508-000 |
| Country |
Brazil |
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| Platforms (1) |
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| Samples (6)
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| GSM830125 |
Sugarcane transcriptome - ch2: Drought_24h_Exp_1; ch1: control_24h_1 |
| GSM830126 |
Sugarcane transcriptome - ch1: Drought_24h_Exp_2; ch2: control_24h_2 |
| GSM830127 |
Sugarcane transcriptome - ch2: Drought_72h_Exp_1; ch1: control_72h_1 |
| GSM830128 |
Sugarcane transcriptome - ch1: Drought_72h_Exp_2; ch2: control_72h_2 |
| GSM830129 |
Sugarcane transcriptome - ch2: Drought_120h_Exp_1; ch1: control_120h_1 |
| GSM830130 |
Sugarcane transcriptome - ch1: Drought_120h_Exp_2; ch2: control_120h_2 |
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| Relations |
| BioProject |
PRJNA148569 |
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