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| Status |
Public on Aug 01, 2012 |
| Title |
Identification of miRNAs regulated by vitamin D within primary human osteoblasts |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Identified a series of miRNAs transcriptionally regulated by vitamin D treatment within osteoblasts. Several miRNAs were validated in dose escalation experiments. Furthermore, downstream miRNA targets were appraised and found to regulate target message and protein levels. These findings were assessed in early and late passaged osteoblasts and found an age-dependent regulation of some miRNAs, while others were considered constituitiously expressed. Inhibitor studies of one key bone-related miRNA demonstrated the importance of this miRNA to regulate vitamin D-mediate osteoblastic differentiation during mineralizaition.
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| Overall design |
Three independent sets of passage 8 primary human osteoblasts were either treated with vehicle (ethanol) or 1,25D3 (10nM) for 6 hours. Total RNA was collected using the miRNA Vana kit (ABI) and then quality assessed, and sent for miRNA array processesing.
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| |
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| Contributor(s) |
Lisse TS, Rieger S, Chun RF, Hewison M, Adams JS |
| Citation(s) |
23362149 |
| |
| Submission date |
Dec 05, 2011 |
| Last update date |
Dec 04, 2015 |
| Contact name |
Thomas Lisse |
| E-mail(s) |
tom.lisse@miami.edu
|
| Organization name |
University of Miami
|
| Department |
Biology
|
| Street address |
1801 Memorial Drive
|
| City |
Coral Gables |
| State/province |
Florida |
| ZIP/Postal code |
33146 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14957 |
Exiqon miRCURY LNA microRNA Array 5th generation (DesignedFor miRBase 14.0, CurrentVersion miRBase 16.0) |
|
| Samples (6)
|
| GSM842862 |
human primary osteoblast passage 8 6 hours vehicle (ethanol) Set1 |
| GSM842863 |
human primary osteoblast passage 8 6 hours vehicle (ethanol) Set2 |
| GSM842864 |
human primary osteoblast passage 8 6 hours vehicle (ethanol) Set3 |
| GSM842865 |
human primary osteoblast passage 8 6 hours treated (1,25D3) Set1 |
| GSM842866 |
human primary osteoblast passage 8 6 hours treated (1,25D3) Set2 |
| GSM842867 |
human primary osteoblast passage 8 6 hours treated (1,25D3) Set3 |
|
| Relations |
| BioProject |
PRJNA150077 |
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