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| Status |
Public on Aug 27, 2007 |
| Title |
Differential regulation of intestinal lipid metabolism in A/J versus C57BL/6J mice during diet-induced obesity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The effects of high-fat (HF) feeding on the gene expression in the small intestine were examined using obesity-resistant A/J mice and obesity-sensitive C57BL/6J (B6) mice. Both strains of mice were maintained on low-fat (LF, 5% fat) or HF (30% fat) diets for two weeks. Oligonucleotide microarray and semi-quantitative RT-PCR analysis revealed that lipid metabolism-related genes including mitochondrial acyl-CoA thioesterase 1, pyruvate dehydrogenase kinase 4, and NADP+-dependent cytosolic malic enzyme were up-regulated by more than 2-fold in both strains of mice. The up-regulated gene expression levels were significantly higher in A/J mice than in B6 mice, suggesting the active lipid metabolism in the small intestine of A/J mice.
Keywords: diet response, strain difference
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| Overall design |
Mice were maintained on the LF or HF diet for two weeks. For each group (n=6), pooled total RNA from two mice of average body weight was used for the analysis.
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| Contributor(s) |
Kondo H, Minegishi Y, Komine Y, Mori T, Matsumoto I, Abe K, Tokimitsu I, Hase T, Murase T |
| Citation(s) |
16822957 |
| |
| Submission date |
Oct 11, 2005 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Hidehiko Kondo |
| E-mail(s) |
kondo.hidehiko@kao.co.jp
|
| Phone |
81-285-68-7769
|
| Fax |
81-285-68-7495
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| Organization name |
Kao Corporation
|
| Department |
Biological Science Laboratories
|
| Street address |
2606 Akabane, Ichikai-Machi
|
| City |
Haga-Gun, Tochigi |
| ZIP/Postal code |
321-3497 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (4)
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| GSM77383 |
C57BL/6J mice fed a high fat-diet |
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| Relations |
| BioProject |
PRJNA93467 |
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