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Series GSE34860 Query DataSets for GSE34860
Status Public on Jan 05, 2012
Title Gene expression profiling in acute myeloid leukemia with mutated NPM
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Approximately one third of acute myeloid leukemias (AMLs) are characterized by aberrant cytoplasmic localization of Nucleophosmin (NPMc+ AML), consequent to mutations in the NPM putative nucleolar localization signal. These events are mutually exclusive with the major AML-associated chromosomal rearrangements, and are frequently associated with normal karyotype, Fms-like tyrosine kinase (FLT3) mutations and multilineage involvement. We report the gene expression profiles of 78 de novo AMLs (72 with normal karyotype; 6 with non-major chromosomal abnormalities) that were characterized for the subcellular localization and mutation status of NPM. Unsupervised clustering clearly separated NPMc+ from NPMc- AMLs, regardless of the presence of FLT3 mutations or non-major chromosomal rearrangements, supporting the concept that NPMc+ AML represents a distinct entity. The molecular signature of NPMc+ AML includes up-regulation of several genes putatively involved in the maintenance of a stem cell phenotype, suggesting that NPMc+ AML may derive from a multipotent hematopoietic progenitor.
 
Overall design 78 de novo AMLs, negative for AML-associated chromosomal translocations at the cytogenetic and/or molecular level.
 
Contributor(s) Alcalay M
Citation(s) 15831697
Submission date Jan 04, 2012
Last update date Nov 14, 2018
Contact name Myriam Alcalay
E-mail(s) myriam.alcalay@ieo.eu
Organization name European Institute of Oncology
Department Experimental Oncology
Lab Functional Genomics
Street address Via Adamello 16
City Milan
ZIP/Postal code 20139
Country Italy
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (78)
GSM856203 Acute Myeloid Leukemia, sample 1
GSM856204 Acute Myeloid Leukemia, sample 2
GSM856205 Acute Myeloid Leukemia, sample 3
Relations
BioProject PRJNA150123

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Supplementary file Size Download File type/resource
GSE34860_RAW.tar 265.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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