|
| Status |
Public on Jan 05, 2012 |
| Title |
Acute Myeloid Leukemia, sample 28 |
| Sample type |
RNA |
| |
|
| Source name |
AML blasts from bone marrow (at diagnosis), mutated NPM
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: AML blasts from bone marrow
set: training
npm status: mutated
fab classification: M5b
flt3 status: D835 mutation
|
| Growth protocol |
RNA was extracted directly from fresh AML blasts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
Images were scanned using an Affymetrix GeneArray Scanner, using default parameters.
|
| Description |
AML_28
|
| Data processing |
The data were analyzed with MASv5 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 04, 2012 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Myriam Alcalay |
| E-mail(s) |
myriam.alcalay@ieo.eu
|
| Organization name |
European Institute of Oncology
|
| Department |
Experimental Oncology
|
| Lab |
Functional Genomics
|
| Street address |
Via Adamello 16
|
| City |
Milan |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE34860 |
Gene expression profiling in acute myeloid leukemia with mutated NPM |
|
| Relations |
| Reanalyzed by |
GSE122505 |
