|
| Status |
Public on Mar 14, 2012 |
| Title |
microRNA and Gene expression profiles in colorectal cancer stromal tissue |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
Using Laser Microdissection System (Leica Microsystems), RNA samples specific for stroma or epithelium were separately collected from thirteen colorectal cancer tissues and four normal tissues. Both microRNA microarray and gene expression microarray were performed in these samples.
|
| |
|
| Overall design |
Tissue samples from thirteen colorectal cancer tissues and four normal tissues were microdissected using Laser Microdissection System (Leica Microsystems), and RNA samples specific for stroma or epithelium were separately collected.MicroRNA and Gene expression profiles obtained from cancer stromal tissues were compared with that of normal colon stromal tissues. we used the commercially available Human Whole Genome Oligo DNA Microarray Kit (Agilent Technologies) for gene expresson analysis and microRNA array for microRNA analysis.
|
| |
|
| Contributor(s) |
Mori M, Mimori K, Nishida N |
| Citation(s) |
22452939 |
| |
| Submission date |
Feb 07, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Koshi Mimori |
| E-mail(s) |
kmimori@beppu.kyushu-u.ac.jp
|
| Phone |
81-977-27-1650
|
| Fax |
81-977-27-1651
|
| Organization name |
Kyushu university
|
| Department |
Department of Surgery
|
| Lab |
Kyushu Univeristy Beppu Hospital
|
| Street address |
4546 Tsurumihara
|
| City |
Beppu |
| State/province |
Oita |
| ZIP/Postal code |
874-0838 |
| Country |
Japan |
| |
|
| Platforms (2) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
| GPL8227 |
Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version) |
|
| Samples (59)
|
|
| Relations |
| BioProject |
PRJNA152525 |
Less...
More...