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Status |
Public on Oct 03, 2012 |
Title |
Pulmonary gene expression in MutaTMMouse orally gavaged with three doses of benzo(a)pyrene and vehicle control |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
In this study, we investigate mRNA profiles in lung of mice exposed to benzo(a)pyrene. Male mice were exposed to three doses (25, 50, and 75 mg/kg/day BaP) for 28 days and profiles were examined three days post-exposure. Our analyses reveal that BaP causes pulmonary specific cellular transformation indicative of carcinogenesis.
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Overall design |
Individual total RNA (200 ng) from 5 mice per treatment group (control, 25mg/kg/day, 50mg/kg/day, and 75mg/kg/day) and universal reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double stranded cDNA.
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Contributor(s) |
Labib S, Williams A, Arlt V, White PA, Halappanavar S |
Citation(s) |
22610609 |
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Submission date |
Feb 10, 2012 |
Last update date |
May 10, 2018 |
Contact name |
Andrew Williams |
Organization name |
Health Canada
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Street address |
50 Columbine
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City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1A OK9 |
Country |
Canada |
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Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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Samples (19)
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Relations |
BioProject |
PRJNA152117 |