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| Status |
Public on Oct 14, 2012 |
| Title |
Transcriptional profiling of somatic cells differentiation throughout oocyte competence acquisition in rainbow trout ovarian follicles. |
| Organism |
Oncorhynchus mykiss |
| Experiment type |
Expression profiling by array
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| Summary |
Somatic cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes).
NC1 samples were collected from late vitellogenic females (LV), C1 samples from post-vitellogenic females (PV), and C2 samples from females undergoing meiotic maturation (Germinal Vesicle Breakdown)
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| Overall design |
Global transcriptional profiling was performed using somatic cells collected from rainbow trout ovarian follicles during in vivo oocyte developmental competence acquisition. Somatic cells were collected at 3 stages of oogenesis: NC1 stage follicles (LV, late vitellogenic, prophase I arrested oocytes, meiotically incompetent and developmentally incompetent, n=6), C1 stage follicles (PV, post-vitellogenic, prophase I arrested oocytes, meiotically competent and developmentally competent, n=16). Ovulatory follicles were also collected during oocyte maturation after in vivo induction (metaphase II arrested oocytes, developmentally fully competent, n=6).
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| Contributor(s) |
Charlier C, Uzbekova S, Bobe J, Chesnel F |
| Citation(s) |
23083410 |
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| Submission date |
Mar 19, 2012 |
| Last update date |
Oct 28, 2012 |
| Contact name |
Julien Bobe |
| E-mail(s) |
Julien.Bobe@rennes.inra.fr
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| Phone |
(33) 2 23 48 57 24
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| Organization name |
Institut National de la Recherche Agronomique
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| Lab |
INRA-SCRIBE
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| Street address |
Campus de Beaulieu
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| City |
Rennes |
| ZIP/Postal code |
35000 |
| Country |
France |
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| Platforms (1) |
| GPL15258 |
Agilent-016320 rainbow trout 37K oligo array [Probe name version] |
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| Samples (28)
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| This SubSeries is part of SuperSeries: |
| GSE36617 |
Contribution of molecular actors from somatic origin to oocyte developmental competence acquisition, lessons from evolution |
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| Relations |
| BioProject |
PRJNA155643 |
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