|
Status |
Public on Apr 16, 2012 |
Title |
Chip-chip from WT and Polycomb Component Knock Out Mouse ES cells for H2AZ, H3K27me3, EZH2 and Ring1B. |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
Summary |
The essential histone variant H2A.Z localises to both active and silent chromatin sites. In embryonic stem cells (ESCs), H2A.Z is also reported to co-localise with polycomb repressive complex 2 (PRC2) at developmentally silenced genes. The mechanism of H2A.Z targeting is not clear, but a role for the PRC2 component Suz12 has been suggested. Given this association, we wished to determine if polycomb functionally directs H2A.Z incorporation in ESCs. We demonstrate that the PRC1 component Ring1B interacts with multiple complexes in ESCs. Moreover, we show that although the genomic distribution of H2A.Z co-localises with PRC2, Ring1B and with the presence of CpG islands, H2A.Z still blankets polycomb target loci in the absence of Suz12, Eed (PRC2) or Ring1B (PRC1). Therefore we conclude that H2A.Z accumulates at developmentally silenced genes in ESCs in a polycomb independent manner.
|
|
|
Overall design |
Array design includes 2 biological replicates for all samples and technical replication (dye swaps for H3K27me3_WT_ES, EZH2_WT_ES, EZH2_RING1b_KO_ES, H2AZ_WT_ES(2), H2AZ_Eed_KO_ES and H2AZ_Suz12_KO_ES). Ring1B_WT_ES is represented by a single replicate.
|
|
|
Contributor(s) |
Illingworth R, Botting C, Grimes G, Bickmore WA, Eskeland R |
Citation(s) |
22496869 |
|
Submission date |
Apr 03, 2012 |
Last update date |
Dec 17, 2012 |
Contact name |
Rob S Illingworth |
E-mail(s) |
robert.illingworth@ed.ac.uk
|
Phone |
01316519640
|
Organization name |
The University of Edinburgh
|
Department |
Centre for regenerative Medicine
|
Lab |
Illingworth
|
Street address |
Centre for Regenerative Medicine
|
City |
Edinburgh |
State/province |
Midlothian |
ZIP/Postal code |
EH16 4UU |
Country |
United Kingdom |
|
|
Platforms (1) |
GPL14936 |
NimbleGen_mouse(MM9)_Hox plus array [Design_ID:11926] |
|
Samples (10)
|
GSM908268 |
H3K27me3 ChIP in WT ES Cells using anti H3K27me3 antibody (Millipore, 07-449) |
GSM908269 |
H3K27me3 ChIP in Ring1B deficient ES Cells using anti H3K27me3 antibody (Millipore, 07-449) |
GSM908270 |
EZH2 ChIP in WT ES Cells using anti EZH2 antibody (Millipore 07-689) |
GSM908271 |
EZH2 ChIP in Ring1B KO ES Cells using anti EZH2 antibody (Millipore 07-689) |
GSM908272 |
H2AZ ChIP in WT ES cells using anti H2AZ antibody (Abcam ab4174) |
GSM908273 |
H2AZ ChIP in Ring1B KO ES cells using anti H2AZ antibody (Abcam ab4174) |
GSM908274 |
Ring1B ChIP in WT ES cells using anti Ring1B antibody (MBL D139-3) |
GSM908275 |
H2AZ ChIP in WT ES cells (Eed+/+ clone 2.21) using anti H2AZ antibody (Abcam ab4174) |
GSM908276 |
H2AZ ChIP in Eed KO ES cells using anti H2AZ antibody (Abcam ab4174) |
GSM908277 |
H2AZ ChIP in Suz12 KO ES cells using anti H2AZ antibody (Abcam ab4174) |
|
Relations |
BioProject |
PRJNA157521 |