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| Status |
Public on Apr 07, 2012 |
| Title |
The Caenorhabditis elegans RDE-10/RDE-11 complex regulates RNAi by promoting secondary siRNA amplification |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
From a forward genetic screen for C. elegans genes required for RNAi, we identified rde-10 and through proteomic analysis of RDE-10-interacting proteins, we identified a protein complex containing the new RNAi factor RDE-11, the known RNAi factors RSD-2 and ERGO-1, as well as other candidate RNAi factors. The newly identified RNAi defective genes rde-10 and rde-11 encode a novel protein and a RING-type zinc finger domain protein, respectively. Mutations in rde-10 and rde-11 genes cause dosage-sensitive RNAi deficiencies: these mutants are resistant to low dosage, but sensitive to high dosage of double-stranded RNAs. We assessed the roles of rde-10, rde-11, and the dosage-sensitive RNAi defective genes rsd-2, rsd-6 and haf-6 in both exogenous and endogenous small RNA pathways using high-throughput sequencing and qRT-PCR. These genes are required for the accumulation of secondary siRNAs in both exogenous and endogenous RNAi pathways.
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| |
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| Overall design |
Small RNA analysis by deep sequencing in various wild type and mutant C. elegans strains.
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| |
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| Contributor(s) |
Montgomery T, Zhang C |
| Citation(s) |
22542102 |
| |
| Submission date |
Apr 06, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Taiowa Montgomery |
| E-mail(s) |
montgomery@molbio.mgh.harvard.edu
|
| Phone |
(617) 643-3319
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Ruvkun
|
| Street address |
185 Cambridge Street, CPZN-7250
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114-2790 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL9269 |
Illumina Genome Analyzer II (Caenorhabditis elegans) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
|
| Samples (19)
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| Relations |
| BioProject |
PRJNA158125 |
| SRA |
SRP012103 |
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