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| Status |
Public on Dec 31, 2021 |
| Title |
In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming (miRNA) |
| Platform organisms |
Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Gallus gallus; Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Remarkable progress has been made in cell fate reprogramming by forced expression of a small number of transcription factors. Major challenges remain, however, in regenerative medicine regarding how to target multiple cell types and direct them to form a functional organ in vivo. Here, we demonstrate that, by changing their stromal microenvironment, adult differentiated cells of endodermal origin can be reprogrammed to generate a functional ectodermal organ. The process of organ regeneration is highly efficient and complete, and depends on epithelial-stromal interactions that lead to successful remodeling of the extracellular matrix and stromal cells that are essential for organ function. Furthermore, it is a multistep process consisting of changes in cell fate, where dedifferentiation occurs more rapidly than redifferentiation, and subsequent morphogenetic reprogramming. Remarkably, neither direct transdifferentiation nor a complete reversion to the pluripotency state is involved in the reprogramming process; instead it features dynamic activities of essential genes that regulate pluripotency and lineage development. Our data have important implications for stem cell biology, cancer biology, and regenerative medicine.
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| |
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| Overall design |
Three replicates of adult and reprogrammed mouse lung and mammary cells were profiled for miRNAs
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| |
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| Contributor(s) |
Sims AH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 16, 2012 |
| Last update date |
Dec 31, 2021 |
| Contact name |
Andrew H Sims |
| E-mail(s) |
andrew.sims@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Institute of Genetics and Molecular Medicine
|
| Lab |
Applied Bioinformatics of Cancer
|
| Street address |
Systems Medicine Building
|
| City |
Carrington Crescent |
| State/province |
Edinburgh |
| ZIP/Postal code |
EH4 2XR |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL13992 |
Agilent-023723 UCSF Multi-species custom 8x15K miRNA array |
|
| Samples (12)
|
| GSM915807 |
Mouse Mammary epithelial cells rep1 |
| GSM915808 |
Mouse Mammary epithelial cells rep2 |
| GSM915809 |
Mouse Mammary epithelial cells rep4 |
| GSM915810 |
Mouse Reprogrammed Lung epithelial cells rep 1 |
| GSM915811 |
Mouse Reprogrammed Lung epithelial cells rep 2 |
| GSM915812 |
Mouse Reprogrammed Lung epithelial cells rep 3 |
| GSM915813 |
Mouse Reprogrammed Mammary epithelial cells rep 1 |
| GSM915814 |
Mouse Reprogrammed Mammary epithelial cells rep 2 |
| GSM915815 |
Mouse Reprogrammed Mammary epithelial cells rep 4 |
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| This SubSeries is part of SuperSeries: |
| GSE37309 |
In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming |
|
| Relations |
| BioProject |
PRJNA159475 |
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