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Series GSE3746 Query DataSets for GSE3746
Status Public on Feb 08, 2007
Title Circadian skeletal muscle_wt and Clock mutants
Organism Mus musculus
Experiment type Expression profiling by array
Summary One hundred ninety wildtype male C57BL/6J mice age 7-10 weeks were purchased from Jackson Laboratory and entrained to a 12:12 light:dark cycle for 2 weeks. Mice were placed in light-tight boxes on a 12:12 LD cycle for 4 weeks, then released into constant darkness. Starting 30 hours after entry into DD (CT18), the left leg muscle from 5 wildtype mice, and the right leg muscle from the same wildtype mice were collected every 4 hours for 48 hours, for a total of 12 timepoints. Total RNA from each side (left or right) were separately pooled to make a single sample for amplification and hybridization. Therefore, duplicate arrays (from left and right side) were performed at each time point. At timepoints 34 through 58 hours in DD, tissues from age-matched male C57BL/6J Clock/Clock homozygous mutant mice that had been treated with the same light entrainment protocol as the wildtype were collected. Tissues were collected from 5 Clock/Clock mutants at each timepoint except for 34 and 46 hours after the onset of DD, when tissues from 10 Clock/Clock mice were collected. As for the wildtype mice, right and left sides were collected, prepared, amplified, and hybridized in separate pools. Mice were sacrificed by cervical dislocation, and the optic nerves were severed in complete darkness; brain dissection was performed using illumination from an infrared viewer (FJW Industries, Palatine, IL). SCNs were dissected out, pooled at a density of 5 per tube in 100 ?l RNAlater (Ambion, Austin, TX), frozen on dry ice, and stored at –80?C until use.
Keywords: time course
 
Overall design duplicates consisted of pools from independent mice
 
Contributor(s) Miller BH, McDearmon EL, Panda S, Andrews JL, Straume M, Walker JR, Esser KA, Hogenesch JB, Takahashi JS
Citation(s) 17360649, 17462724, 17550994
Submission date Dec 06, 2005
Last update date May 21, 2019
Contact name John R Walker
E-mail(s) jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platforms (1)
GPL1073 GNF1M
Samples (38)
GSM86176 CT18A;skeletal muscle;NONE;C57/BL6;30517
GSM86177 CT18B;skeletal muscle;NONE;C57/BL6;30518
GSM86178 CT22A;skeletal muscle;NONE;C57/BL6;30519
This SubSeries is part of SuperSeries:
GSE3751 Circadian rhythm in clock mutants
Relations
BioProject PRJNA94305

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3746_RAW.tar 215.7 Mb (http)(custom) TAR (of CEL)

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