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Sample GSM86196 Query DataSets for GSM86196
Status Public on Feb 08, 2007
Title CT58A;skeletal muscle;NONE;C57/BL6;30537
Sample type RNA
 
Source name CT58A;skeletal muscle;NONE;C57/BL6;30537
Organism Mus musculus
Characteristics CT58A;skeletal muscle;NONE;C57/BL6;30537
Extracted molecule total RNA
Extraction protocol Tissues were collected from 5 Clock/Clock mutants at each timepoint except for 34 and 46 hours after the onset of DD, when tissues from 10 Clock/Clock mice were collected and run as independent replicates. Mice were sacrificed by cervical dislocation, and the optic nerves were severed in complete darkness; brain dissection was performed using illumination from an infrared viewer (FJW Industries, Palatine, IL). SCNs were dissected out, pooled at a density of 5 per tube in 100 ?l RNAlater (Ambion, Austin, TX), frozen on dry ice, and stored at –80?C until use.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures except for use of a custom 24-channel fluidics machine
Scan protocol standard Affymetrix procedures
Description Circadian muscle48hr_gcrma
Data processing gcRMA
 
Submission date Dec 06, 2005
Last update date Feb 08, 2007
Contact name John R Walker
E-mail(s) jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL1073
Series (2)
GSE3746 Circadian skeletal muscle_wt and Clock mutants
GSE3751 Circadian rhythm in clock mutants

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 11122.26
AFFX-18SRNAMur/X00686_5_at 15407.56
AFFX-18SRNAMur/X00686_M_at 3307.136
AFFX-b-ActinMur/M12481_3_at 50863.35
AFFX-b-ActinMur/M12481_5_at 66267.87
AFFX-b-ActinMur/M12481_M_at 6497.734
AFFX-BioB-3_at 1366.355
AFFX-BioB-5_at 1683.562
AFFX-BioB-M_at 4723.17
AFFX-BioC-3_at 4789.197
AFFX-BioC-5_at 3942.25
AFFX-BioDn-3_at 27825.07
AFFX-BioDn-5_at 8741.689
AFFX-CreX-3_at 158392.7
AFFX-CreX-5_at 154743.9
AFFX-DapX-3_at 89.97614
AFFX-DapX-5_at 118.0047
AFFX-DapX-M_at 93.928
AFFX-GapdhMur/M32599_3_at 269198.5
AFFX-GapdhMur/M32599_5_at 266495.5

Total number of rows: 31373

Table truncated, full table size 735 Kbytes.




Supplementary file Size Download File type/resource
GSM86196.CEL.gz 4.8 Mb (ftp)(http) CEL

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