|
Status |
Public on Feb 08, 2007 |
Title |
CT58A;skeletal muscle;NONE;C57/BL6;30537 |
Sample type |
RNA |
|
|
Source name |
CT58A;skeletal muscle;NONE;C57/BL6;30537
|
Organism |
Mus musculus |
Characteristics |
CT58A;skeletal muscle;NONE;C57/BL6;30537
|
Extracted molecule |
total RNA |
Extraction protocol |
Tissues were collected from 5 Clock/Clock mutants at each timepoint except for 34 and 46 hours after the onset of DD, when tissues from 10 Clock/Clock mice were collected and run as independent replicates. Mice were sacrificed by cervical dislocation, and the optic nerves were severed in complete darkness; brain dissection was performed using illumination from an infrared viewer (FJW Industries, Palatine, IL). SCNs were dissected out, pooled at a density of 5 per tube in 100 ?l RNAlater (Ambion, Austin, TX), frozen on dry ice, and stored at –80?C until use.
|
Label |
biotin
|
Label protocol |
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures except for use of a custom 24-channel fluidics machine
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Circadian muscle48hr_gcrma
|
Data processing |
gcRMA
|
|
|
Submission date |
Dec 06, 2005 |
Last update date |
Feb 08, 2007 |
Contact name |
John R Walker |
E-mail(s) |
jwalker@gnf.org
|
Phone |
858-812-1636
|
Organization name |
Genomics Institute of the Novartis Research Foundation
|
Lab |
Genetics Core
|
Street address |
10675 John Jay Hopkins
|
City |
San Diego |
State/province |
CA |
ZIP/Postal code |
92121 |
Country |
USA |
|
|
Platform ID |
GPL1073 |
Series (2) |
GSE3746 |
Circadian skeletal muscle_wt and Clock mutants |
GSE3751 |
Circadian rhythm in clock mutants |
|