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| Status |
Public on Dec 25, 2019 |
| Title |
Histone H3 lysine 56 acetylation is required for formation of normal levels of meiotic DNA breaks in S. cerevisiae |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Genomic profiling of (1) histone H3K56 acetylation and (2) meiotic DSBs flanked by replication protein A (RPA). The major goal was to reveal the genome-wide distribution DSBs when the H3K56 residue was mutated to alanine (H3K56A).
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| Overall design |
Meiotic DSBs were mapped in control (H3 ctrl) and H3K56A mutant plasmid shuffle strains using the replication protein A (RPA) ChIP approach (Borde V et al. 2009 (PMID 19078966)) at 4 hours after the induction of sporulation. H3K56 acetylation were mapped by ChIP-chip using a specific antibody agains this histone modification.
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| Contributor(s) |
Székvölgyi L |
| Citation(s) |
31998719 |
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| Submission date |
Apr 23, 2012 |
| Last update date |
Dec 19, 2022 |
| Contact name |
Lorant Szekvolgyi |
| Organization name |
University of Debrecen
|
| Department |
Department of Pharmacy
|
| Lab |
Genome Architecture and Recombination Research Group
|
| Street address |
Egyetem ter 1.
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| City |
Debrecen |
| ZIP/Postal code |
4032 |
| Country |
Hungary |
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| Platforms (1) |
| GPL4131 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) |
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| Samples (3) |
| GSM4138653 |
Meiotic DSBs mapped in a control (H3) plasmid shuffle strain |
| GSM4138654 |
Meiotic DSBs mapped in a H3K56A mutant plasmid shuffle strain |
| GSM4138655 |
H3K56 acetylation in meiosis mapped in a wild type strain |
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| Relations |
| BioProject |
PRJNA161023 |
